Studies On The Germplasm Innovation Of Soybean (Glycine Max L.) Cyst-nematod Resistance Breeding

As an important oil crop, grain and forage crop, with demand exceeding supply and sharp increase in imports, the soybean industry is facing serious challenges in China. Soybean Cyst Nematode is one of the most important plant disease in soybean output. Now strategies to manage nematodes are based on crop rotation, nematocides, biological control, use of resistant cultivars etc, but inadequate. With the further investigation of molecular interaction between plant and nematode, and the development of molecular genetic technology, it has been a hot spot in looking for novel ways to prevent nematode by using genetic engineering technology.The expression vector of Hs1pro-1cloned by German scientists was constructed and transformed into soybean cotyledon node with Agrobacterium-mediated to get transgenic plants. This study also appraisals Hs1pro-1transformation plants using PCR method, bar quick dip stick detection method and leaf painting using135mg·L-1glufosinate. Three kinds of identification methods consistently show39T0positive transgenic events. Three kinds of identification methods are consistent.The results suggested that leaf painting using135mg·L-1glufosinate could be an effective and costless method to detect genetically modified soybeans progeny using bar gene as a selective agent.In this study the expression of the target gene in T1plants was detected by semi-quantitative RT-PCR and the gene in positive lines was expressed. Identifying T2lines with susceptical cultivar Lee and resistant cultivar as control, the result indicated the positive lines have certin resistance to SCN.