Interaction Of Glucose On Lactose Bio-synthesis In Dairy Cow Mammary Epithelial Cells

Low milk yield, low milk fat percentage and protein content and high incidence of mastitisare serious problems in Chinese dairy industry, which are the important factors limit thedevelopment of dairy industry of our country. So, how to improve the quality of milk efficientlyand safely has became an important proble of dairy industry. The study of Regulates the Glucoseand the Lactose Bio-synthesis Function Gene of the Lactation Progress in the Mammary Glandcould provide theoretical base and practical guidance in improving of milk production and milkquality in dairy cow, which has extremely important value of theory research and application.The glucose precursors necessary for the synthesis of lactose in the lactating mammary gland,is the maintenance of animal life and to ensure that one of the nutrients necessary for theproduction. Lactose is an important regulator of breast intake of water, the main factor determiningmilk production. On the one hand to provide energy for the cell metabolism, on the other hand itsmetabolic intermediates can also be used as a synthetic substrate of protein and fat, and only toensure an adequate supply of glucose to be able to operate normally, the function of the animal’sbody. Most of the glucose concentration of starch rumen-protected and glucose balance supply, buthow to control the lactose synthesis thereby regulating the lactation is still rarely reported. Only byunderstanding the mammalian glucose lactose synthesis in the lactation process to be a reasonableregulation, so as to achieve the purpose of increase milk production and improve milk quality.Our work was divided into two parts. The fist part as follows: CASY was used to study thecell viability and proliferation treated with, glucose optimal concentration and time; qRT-PCR andWestern blotting was used to detect the impact of signal molecules associated with lactation inDCMECs treated with, Lactose synthesis functional genes. The second part as follows: Of AKT1gene silencing experiments to determine the optimal silence, and to add glucose concentration wasdetermined by CASY law DCMECs vitality; using qRT-PCR and Western blotting the methoddetection DCMECs in lactose synthesis gene. The results showed that:1) The glucose can significantly improve the vitality and proliferative capacity of DCMECs,the glucose concentration of the best role of DCMECs12mmol/L, the best role of time is24h;2) Glucose Related Gene regulation for lactose synthesis. qRT-PCR method to detect results showed GLUT4, GLUT12mRNA expression levels of α-LA lower level of mRNA expression ofAKT1, GLUT1, HKII and β-1,4-GT higher; application of Western blotting to detect glucose theAKT1, GLUT1, HKⅡ, β-1,4-Gal T, SLC35A2and SLC35B1the expression levels compared tothe control group were significantly upregulated (P<0.05).3) The glucose can by DCMECs needs adjustment. AKT1gene silence, lactose synthesis genesignificantly reduced, the best time of silence for48h, the best demand for glucose with12mmol/L.