Rice Rf-1Gene Infertility BT Type Cytoplasmic Fertility Restoration Research

Rice is one of the most important food crops. The breeding objectives that high-yield,high-quality, disease resistance and adaptability are pursued by breeders. In this study, fertilityas a breakthrough, select Japanese sunny, station95and other rice material from geneticallymodified, hybridization, rapprochement and other breeding methods, exploration a fertilityrole in the cultivation of new varieties, made the following main results:1. An Agrobacteriumtumefaciens binary vector that carry the Rf-1gene fragment，ctG16-SUB6.8.77independent transgenic lines by hygromycin detected, Rf-1gene detection,seed setting rate research, to prove Rf-1gene had been integrated into the rice genome. Thenthe Rf-1gene had been transferred to some new rice varieties.2. Using long PCR technology, combined with the re-sequencing, compared Nipponbareand Xiaozhan95genome structure. The results show that the mode varieties, the Nipponbarehave the typical japonica genome structure, the Xiaozhan95show on inversion and missingseven genes of the transcription factor genes on the chromosome5. On chromosome10,Nipponbare and Xiaozhan95have a significantly different in structure. The polymorphisms(SNPs) to2.48%-6.25%(EcoRI sites). PCR amplification results from long to see, theNipponbare and Xiaozhan95have a higher polymorphism.3. Innovation indica-japonica hybridization and the resumption of diplomatic relations,15new germplasm, H1381, H138, Jindao44and other varieties or a combination ofproduction at or close to the current level of super rice, integrated traits has been markedlyimproved.(1) H294plant height105cm, panicle weight4.7g,9tillers and yield per plant40.2g;(2) H138plant height102cm, panicle weight5g,13tillers;(3) H1381plant height116cm, panicle weight5g,11tillers, indica and japonica two genes with a reasonable,japonicaclinous type. Above new germplasm, new combination to meet or exceed the currentsuper rice production levels, better quality, better resistance, adaptability.Studies have shown that fertility as a breakthrough, combine to create an ideal plant typeand create the ideal population structure, is a new way of breeding new varieties to cultivatehigh-yield, quality, disease resistance and adaptability.