Subcellular Localization And Functional Analysis Of Fructose-1,6-bisphosphate Aldolase Gene From Populus Euphratica

The fructose-1,6-bisphosphate aldolase gene (PeALD) of Populus euphratica was cloned and constructed to an expression vector pGEX-4T-1. And then the recombinant plasmid pGEX-PeALD was transformed into Escherichia coli competent cell BL21(DE3) to identify the expression of fusion protein GST:PeALD that induced by IPTG. The SDS-PAGE gel electrophoresis showed that the molecular weight of fusion protein was about52KDa.The E. coli strains expressing the PeALD showed a higher salt tolerance compared to control strains that expressing the null vector. We constructed the fusion expression vector PeALD:GFP and identify the localization of PeALD in Arabidopsis mesophyll protoplast. The green fluorescence detected by fluorescence microscope showed that the protein encoded by PeALD localized in the cytoplasm. Seed germination experiment under200mmol· L-1NaCl showed that genetically modified tobacco seeds exhibited a higher salt tolerance. The content of soluble sugars in wile-type and transgene lines was analyzed after salt treatment. Results showed that the content of glucose in transgenic plants was greatly elevated under salt stress. Our data suggest that PeALD enhanced plant salt adaptation through up-regulation of glycolysis and aerobic respiration.