Analysis Of Alternative Splicing In Whole Transcriptome And Its Regulator SR Protein Under Drought Stress In Seed Of Zea Mays L

In this manuscript we will to explain the alternative splicing of the whole transcriptomes in seedling stage.under drought stress in two maize (zea mays sp. L) inbred lines, the sensitivity to drought stress inbred lines Ye478(from8112and5003of the system, the most popular inbred line in China) and drought tolerance oneH21(from the P78599, pioneer Seed Company, USA, widely plant in Northwest China and half arid zones).Drought stress treatment is in the seedling stage, analysis the data of alternative splicing in whole transcnptomeobtained using high-throughput Solexa sequencing for gene expression, through the comparison of differences of genes alternative splicing events and degree under drought stress and normal condtion, to understand the variable shear biological and molecular pathway; by means of reverse transcription PCR amplified part of splice variants of some genes; by high-throughput sequencing analysis and qRT-PCR analysis to understand the expression of corn important regulatorsof alternative splicing, SR protein gene family. Screening of drought related candidate SR members, and reveal the internal relationship between its response gene expression and regulation of maize drought stress. The follows are the result:1. Overall, drought stress will be alternative splicing induced gene reflect the drought degree of Maize.Drought stress physiological state of the plant will have an impact, all kinds of plant body and water related physiological and biochemical changes. Ye478because of the drought resistance ability is far lower than the H21, so the number of alternative splicing occurs under drought stress than drought tolerant lines H21. Affected by drought, plant alternative splicing number will rise by environmental stresses affecting plant will increase the diversity of genome is greatly increased in addition, alternative splicing diversity variable change to some extent reflects the corn stress influence degree; alternative splicing drought-tolerant strains H21occurred in drought conditions change to less than Ye478, due to alternative splicing can influence gene transcription of the fate, it can be understood as Ye478affected by drought is more serious, shut down the expression of a large number of required for non housekeeping genes.2. Alternative splicing has certain influence on maize drought adaptability. There are some difference variable under the condition of drought stress between H21and Ye478:alternative splicing genes could increase maize drought tolerance similar alternative splicing genes in H21and Ye478drought stress while it occurs alternative splicing in H21control group, may be associated with Maize Drought adaptability.3. In all two maize inbred lines, most alternatively splicedgenes occurAlternative splicingin both drought and non-drought stress occurred alternative splicing.2/3in H21and4/5in Ye478alternatively spliced genesin normal moisture conditions still alternative splicing under drought stress. Induced by drought and alternatively spliced genes accounted for about Ye478and H21drought all alternatively spliced genes in1/3, shows a portion of a gene alternative splicing is maize physiological functions necessary, are not affected by the drought and other environmental factors.4. The majority of the alternatively spliced genes under drought stress related pathways in NTP binding, sugar metabolism and protein synthesis, suggesting that transport, alternative splicing occurs in Maize under drought stress genes were mainly protein and energy metabolism related genes. This indicates that the protein and energy metabolism is the main physiological pathway in Maize under drought stress, the stress of drought resistance of important pathways associate with Maize under drought stress.5. Magnesium ion binding protein is an important member of H21under drought stress specific alternatively spliced proteins, suggesting that the magnesium ion binding splicing protein may be associated with drought resistance for H21.6. The ABA signal transduction pathway of Maize under drought stress, is triggering main transmission of the plant stress response. Induction of ABA protein plays an important role in plant abiotic stress. ABA induced protein, either in H21or in Ye478ocurrs a large number of alternative splicing due to drought. Many transcription factorshave similar alternative splicing changes in the H21and Ye478under drought condition. In addition, R protein and splicing factor family also have alternative splicing, these alternative splicing has been verified by experiment.7. The SR protein is one of the most important splicing factors in maize, the change trend of whole genome alternative splicing, is consistent with the trend of SR splicing factors expression; whole genome splicing changes can be replaced by the expression of a large number of SR splicing factors, so it can be used to change the amount of splicing factor expression to express the maize drought tolerance.8. The SR protein family member and the stress response is closely related to maize. RSZ, ASF/SF2families may be associated with maize cell apoptosis can reflect the corn stress to a certain extent, and the different expression of some members of the SC35subfamily of transcription in each group shows its close relationship with maize drought tolerance.