Clostridium Perfringens And Trueperella Pyogenes Immunological Evaluation And Two Of The Preparation Of Inactivated Vaccines

Clostridium perfringens disease is also known as the "sudden death", in recent years, ourcountry many farm break out Clostridium perfringens disease, it’s year-round disease, highincidence in the summer two season. The disease has been widely spread in the world, a globalepidemic situation, serious harm to national livestock and poultry industry development, the mainuse of antibiotics and inactivated vaccine to control the disease, often caused by pyogenic infectionin multiple organs of Trueperella pyogenes infections, mainly pneumonia, mastitis, subcutaneousabscess, caused huge losses for animal husbandry in China, because of the lack of effectivevaccines, antibiotics at present is mainly for its control.For the effective control of Clostridium perfringens type A and Trueperella pyogenes diseaseoccurrence and epidemic, Trueperella pyogenes strains used in this study. The bovine Clostridiumperfringens type A virulent strain C987and the cow was isolated as the basis, establish thepathological infection model, influence, Propolis Adjuvant alhydrogel adjuvant, chitosan on theimmune effect of the vaccine compared, successfully developed the different bacterial suspensionratio of two vaccine, and to evaluate its immunological effect.The specific antibodies were detected by ELISA method, two kinds of bacterial vaccineshowed alhydrogel on mice immune response ability in Propolis Adjuvant group and chitosanadjuvant group, immunity protective test results also confirmed, alhydrogel adjuvant vaccine twobacterial immune protection to mice rate higher than other adjuvant vaccine group, but theTrueperella pyogenes aluminum adjuvant vaccine can not provide100%protection in mice. So bythe two antigen in different proportion, to enhance the immune effect of Trueperella pyogenesvaccine. The results showed:1:4mixed vaccine group PLO protein specific antibody level was1:1,1:2mixed vaccine group, but no significant difference (P>0.05), intraperitoneal inoculation of2LD50Trueperella pyogenes bacteria,1:4vaccine can give mice provide100%protection force;1:4mixed vaccine group alpha toxin protein antibody level is slightly lower than1:1, the1:2mixingratio of the vaccine group, but the difference was not significant (P>0.05). Subcutaneousinoculation of2LD50Clostridium perfringens bacteria,1:1,1:4vaccine group can give miceprovide100%protection force. Based on the above considerations, the final Clostridiumperfringens and Trueperella pyogenes two vaccine optimum mixing ratio for1:4antigen, immuneadjuvant for alhydrogel.