Cloning And Expression Analysis Of Sucrose Transporter Related Gene Of Sugarcane

Sugarcane is an important sugar crop, and a high effective photosynthesis C4 species. The accumulation of phenomenal concentrations of sucrose by sugarcane (Saccharum officinarum L.)has been the focus of intense study.Sucrose is the major carbon compound translocated in sugarcane.During transport from source organs to sink organs,sucrose either follow a symplasmic pathway,moving from cell to cell via plasmodesmata,or move apoplasmically through the cell walls and intercellular spaces of the tissues.The transport pattern depends on the tissue type and stage of development. Transfer of sucrose between the apoplasmic and the symplasmic compartments is mediated by transporter proteins located in the plasma membrane with proton electrochemical potential difference of H+-ATPase forming.ShVHA-C and ShSUT2 has been isolated from sugarcane by Rapid Amplification of cDNA Ends (RACE) technique. Sequences analysis showed that ShVHA-C full-length cDNA is 1312 bp, include an 1056bp ORF (3-1058bp), code 351 amino acids, ShVHA-C is 94.10% identity with oryza sativa VHA-C;ShSUT2 full-length cDNA is 1906 bp, include an 1524bp ORF (37-1560bp),code 507 amino acids, and has typical 12 integrity transmembrane helices.ShSUT2 is 72.25% identity with Sorghum bicolor repeat protein SUT2.Furthermore,the spatial expression pattern of ShVHA-C and ShSUT2 was studied by RT-PCR analysis in roots, stems and leaves of sugarcane ROC22.ShVHA-C and ShSUT2 was detected expression in every tested tissue,and ShVHA-C and ShSUT2 expression in leaves was the highest, and lowest in roots.In conclusion, we presumed that ShVHA-C and ShSUT2 was correlated with sucrose transport and accumulation in sugarcane. All these results would provide valuable information for elucidating the regulatory mechanism of sucrose accumulation in sugarcane.