Research On Improving Saccharinity Of Sugarbeet By Sucrose Transporter Gene

Sucrose is the main translocation form of carbohydrate in plants. Sucrose translocation is very important to the growth and development of plants. Transport of sucrose from the source organ to the sink organ is mediated by sucrose transporters in higher plants. In agricultural production, in order to obtain high yield ,not only to enhance the formation of the biological yield of photosynthesis, but also to the adoption of certain measures to improve production economics. Therefore, separation and identification of higher plant sucrose transporter and study of its function not only has profound theoretical significance, but also to improve the economic yield of crops is of great practical value.Sugar beet as biennial plants and xenial crop, cultivated and domesticative time is short. Genetic background is straitness and resource is scarce. Especially, what self-crossing is highly on appetency makes easy lose of good single genotype, which propagatation and conservation of good varieties breeding brings great difficulty. Plant tissue can provide a new way for sugarbet breeding. At present, studies on sugar beet tissue culture show that organogenesis regeneration mode was more suitable for the regeneration of seedings as a foreign gene into the receptor plant.In this experiment ,according to the role of sucrose transporter sugar in the source accumulation, from the point of view of controlling the relationship between the Treasury and source , using means of molecular biology, cloned AtSuc3 and AtSuc4 genes from Arabidopsis and cloned VvSuc11 and VvSuc12 genes from the grapes. The promoter of sweet potato root-specific storage protein gene was used and five plant expression vectors were constructed: pBI2301-Q3- S3, pBI2301-Q3- S12, pBI2301-Q4- S4, pBI2301-Q4- S11 and pBI2301-Q3- S3-Q4- S4.Sugar beet regeneration was established by non-experimental seeding, preculture, induction of differentiation. Result indicated that sugar beet seeding can growth and adventitious buds can be induced on ms pre-culture medium additional 6-BA and NAA. The bud induction rate was 50%. The induction rate of root on MS attached a certain amount of IBA was more than 75%. Transplant survival rate was as high as 95%. Seed germination medium: (1)1/2MS. Pre-culture medium: (2) MS+6-BA0.5mg/L+NAA0.05. Adventitious bud induction medium: (3) MS +6- BA0.5 + NAA0.05. Rooting medium: (4) MS + IBA2.0.The construction of plant expression vector for transformation of sugar beet is of great significance for the further study of transporter function of sucrose transporter gene in the regulation of genetically modified sugar beet.