Cloning Of The Hypoxia Inducible Factor-1α And Gene Expression Analysis Of Pelteonagrus Fulvidsco

Hypoxia inducible factor -1 (hypoxia inducible factor-1, HIF-1) is a overall regulatory factor in hypoxia response, which is a Heterodimeric transcription factor composed of two Homologous subunit: HIF-1αis the functional unit, which is the unique subunit of hypoxia inducible factor-1. The expression of protein is fine-tuned by oxygen partial pressure. Pelteonagrus fulvidsco HIF-1αgene is 2628 bp encoding 795 amino acid, predicted molecular weight is 88.5448KD and PI is 5.01. The sequence of the Pelteonagrus fulvidsco HIF-1αdetermined the quality of the protein: it is a kind of unstable secreted protein, with about seven hydrophobic regions, without transmembrane region and signal peptide, containing three nuclear localization signal. Pelteonagrus fulvidsco HIF-1αprotein contains three conserved N-functional domains: HLH、PAS-A and PAS-B. These three domains contain multiple functional sites. The tertiary structure of Pelteonagrus fulvidsco HIF-1αis with high homologyof other HIF-1α. Under normal oxygen condition. There is a relative highest expression of HIF-1αin the Pelteonagrus fulvidsco liver, followed by kidney, the least expression level was found in Gill. After induced by hypoxia (the concentration of oxygen is 5ppm), the level of HIF-1αexpression were significantly increased in all the tissues espicially in kidney. Western Blot results showed that in normal oxygen conditions, there is no Pelteonagrus fulvidsco HIF-1αprotein band; but in hypoxic conditions, the protein band is clear.