The Molecular Mechanism Of Mice To The RNV By Hypoxia And Experimental Study On KH902 To The RNV And CNV

The probability of animal tumors and eye diseases were increased. The study of these diseases have continued to increase for protecting and the preventing these diseases. Tumor neovascularization and eye diseases are closely linked. Endothelial growth factor (VEGF) in retinal and choroidal neovascularization play a key role in the occurrence. Firstly ,the main purpose of this study is to investigate the the process of hypoxia-inducible factor (HIF) and vascular endothelial growth factor (VEGF) expression changes and the expression of the relationship between them in occurrence of retinal neovascularization. Secondly, the purpose of this study is to observat and detect the effect that KH902 inhibit angiogenesis. It lay a foundation for treatment of these diseases and conservating and utilizating the experimental animal genetic resources . The main test results are following.1. RT-PCR detect the expression of VEGF and HIF of the high oxygen treatment group and normal control group of mice. Results show in the model mice eyes, the expression of VEGF and HIF were up-regulated gradually after returned to the room air. During P13 to P18, a 44.4% to 143% and a 197% to 255% increasing were obtained in the level of VEGF and HIF respectively in the RNV model mice eyes compared with that in the normal control ones. (p<0.05 ) The results of blot detection of VEGF protein expression show that the expression of VEGF protein high oxygen treatment group accounts protein expression 139% (group 1) and 143% (Group 2)for the normal control group after 5 days out of the oxygen room.2. After the retina of mice piece, we observe fluorescein leakage on neovascularization in the difference between the inverted fluorescence microscope. The results showed that each slice of membrane of vascular endothelial cells of neovascularization is 0.04±0.20 in the normal control group of 380 sections. The results showed clearly the growth of new blood vesselsat the same time also created a successful model of neovascularization.3. We observe neovascularization on fluorescein leakage though the retina of mice piece. The results showed that fluorescence in the normal control group of vascular leakage is rare, and high-oxygen group deal with a lot of fluorescein leakage . Fluorescein leakage in the vessel wall because the new structure is not very complete and fluorescein can be leakage of blood from the newborn. We can use the number of fluorescent leakage as a standard to determine the extent of neovascular growth.4. After injected KH902 to model Mouse, we observat fluorescence leakage of internal limiting membrane of retinal blood vessels of the vascular endothelial cells and retinal vascular through HE staining. We compare model to evaluate KH902 on neovascularization.5. The monkey eye be funded laser irradiation to eye injuries and then injected by the sight glass, such as the size of peptide or PBS (groups). After fundus imaging, fluorescein leakage can be observed. The results showed that fluorescence leakage is in serious condition in group PBS. Four eyes of 25 points have 22 laser fluorescence leakage points. Results show that KH902 inhibiting choroidal neovascularization of monkey be laser-induced have significant role.