Synthesis Of 6-arylazo-7-hydroxy-3-methyl-1-phenyldipyrazolo [5, 1-c:3’, 4’-e] [1, 2, 4] Triazines

Objective There are strme differences in the nucleic acid between human normal cells and cancer cells, therefore it is a method to research anti-cancer drugs thai changing the structure of the basic group of the nucleic acid to search nucleic acid antagonists. In this subject, we designed and synthesized purinc analogues, expecting to screen high selective and active leading compounds that have anti-cancer effects. Methods Prom ethyl cyanoacetate, we synthesized the key intermediates 5-amino-4-arylazo-3-hydroxy-1H-pyrazoles through hydrazinolysis. cyclization and diazo-coupling, and then synthesized the objective compounds 6-arylazo-7-hydroxy-3-methyl-1-phenyldipyrazolo[5,1-c:3’,4’-e][1.2,4] triazines through diazo-coupling and intramolecular cyclization. And the structures of these compounds were identified by TLC, elemental analysis. IR and 1H-NMR. Result twelve new purine analogues were synthesized. Conclusion The resulting compounds were all confirmed to be the objective compounds by elemental analysis. 1R and 1H-NMR.The pharmacological test for these compounds is being carried out!...