Selection Of DNA Aptamers Against Glioblastoma Multiforme Cells With High Affinity And Specificity

Objective: To select aptamers that can be able to specifically bind to humanglioblastoma multiforme cells by using cell-based Systematic Evolution ofLigands by Exponential enrichment (cell-SELEX).Methods: Initial DNA library with84bp length was chemically synthesized. Usehuman glioblastoma multiforme cell line U118-MG as target cells, and humannormal astraglial cell line SVGp12as control cells to generate aptamers thatcan be able to specifically bind to U118-MG cells by using cell-SELEX. Flowcytomitry and confocal microscopy were introduced in order to investigatethese aptamers’biological properties.Results:1. We generated two target-specific aptamers named GBM128and GBM131against cultured human glioblastoma multiforme cell line U118-MG after30rounds selection. These two aptamers have high affinity and specificity againsttarget glioblastoma multiforme cells.2. These two aptamers can perfectly bind to the target cells in complicatedbiological environment. The target of the aptamers is a membrane protein onthe cell surface.3. These two aptamers can bind to different clinical glioma tissues but notnormal brain tissues.Conclusion: Aptamers GBM128and GBM131can specifically bind to the targetmembrane protein. The selected two aptamers could be used to identifyspecific glioblastoma multiforme biomarkers. Methods of molecular imaging,targeted drug delivery, ligand guided surgery can be further developed basedon aptamers.