Applied The Expression Of Carbonic Anhydraseâ…¡ And Aquaporin1/4to Explain The Significance Of The Relationship Between "the Gas And The Body Fluid" In The Pathological Mechanism Of "Disease Of Lung Affecting Large Intestine"

―Lung being connected with large intestine" first appeared in the―HuangdiNeijing", which is one of the basic theories of traditional Chinese medicine(TCM). Now the understanding of its modern mechanism is mainly reflected inthe following areas: embryonic sources, public mucosal immunity, the gasdischarge in the endocrine substances, nervous system, intestinal endotoxemia,nervous-endocrine-network systems and other aspects.TCM believes that the―lung being connected with large intestine" inpathological change mainly issued in the air-less and easy to dry evil injuredaspects of that―the gas" and―the body fliud", this points will be studied. Methods: Used improved smoke-induced method to prepare rat model ofchronic bronchitis; used the ratio of wet and dry weight to detect fecal watercontent; used ELASA to detect vasoactive intestinal peptide (VIP) and substanceP (SP) content of plasma, bronchoalveolar lavage fluid (BALF), lung and colontissue homogenates; applied immunohistochemistry and western blot analysis toobserve the expression of AQP1/4and CA II in lung and colon tissues.Trying to explore the relationship between―the body fluid of lung" and―thebody fluid of large intestine"; the relationship between―the gas of lung" and―the body fluid of large intestine". To explain the pathological mechanism of―disease of lung affecting large intestine", provide a basic guide for clinicaltreatment.Results: Compared with the control group, the fecal water content of modelgroup rats increased significantly (P<0.05); AQP1/4expression in the lungtissue decreased significantly (P<0.05); AQP4expression in colon tissuesignificantly decreased (P<0.05). The content of VIP and SP in plasma, BALF,lung and colon tissue homogenates had no significant difference (P>0.05).Compared with the control group, the CAII content of BALF of model groupwere significantly reduced (P<0.05), CAII expression in lung tissue wasdecreased significantly (P <0.05). Compared the two groups, the expression ofCA II in the colon tissue, the CA II content of plasma and lung and colon tissuehomogenates was no significant difference (P>0.05).Conclusion:1. AQP1/4is the potential molecular basis of the relationship between―thebody fluid of lung" and―the body fluid of large intestine" of―disease of lung affecting large intestine". CA II may be one of the foundations ofmolecular biology of―Lung dominate gas, control respiration", but it’s roleof the relationship between―the gas of lung" and―the gas of largeintestine" of “disease of lung affecting large intestine" need to beconfirmed by further studies.2. CA II and AQP1/4may be the potential molecular basis of the relationshipbetween―the gas of lung" and―the body fluid of large intestine" of―diseaseof lung affecting large intestine".3. In this study, the content of VIP and SP of plasma, BALF, lung andproximal colon tissue homogenates had no significant difference betweentwo groups. We speculate the reason may be model specific or lesionsinvolving the extent relevant, pending verification step forward.4. The relationship between AQPs and VIP or SP, and the relationshipbetween CAs and VIP or SP to be after further argument, but also providenew idea for the future study about the biological mechanisms of―Lungbeing connected with large intestine".5. Will be applied drug intervention in order to verify the pathologicalmechanism of―disease of lung affecting large intestine" is the focus of ourfuture research.