The Basic And Clinical Research Of Postoperative Infection (Bacterial Biofilms) After Cochlear Implantation

Objectives：To construct the bacterial biofilm animal model after the infection ofthe cochlear implantation and morphological observationï¼›observe thestaphylococcus epidermidis in vitro and in vivo of the bacterial biofilmformation，morphological structure model，and prove its strong pathogenicï¼›to explore treatment measures of eradicating and inhibiting bacterialbiofilm formation.Methods：To build staphylococcus epidermidis biofilm in vitro model.Usingscanning electron microscopy （SEM） in New Zealand rabbits CIpostoperative infection BBF model in vivo.Results：①Staphylococcus epidermidis biofilm formation in vitro wassuccessfully established, with Congo red medium BBF can early detectionof screen;â‘¡We built the S.E.biofilm in vivo successfully that was NewZealand rabbit animal model of flap infection with cochlear implantation；③The scanning electron microscopy （SEM） successfully observed thetypical BBF morphological structure. bacteria fiber kind material betweenmutual adhesion, bacteria form, spherical staphylococcus epidermidisclouds distribution, gathered together, a multi-layer cell structure,fiber appearance glycosaminoglycan and bacteria the links between, hypha,the extracellular matrix （ECM） mutual crisscross, a compact film mesh ofthe space structure;â‘£The use of alternative antimicrobial agents suchas H₂O₂, strong iodine could eradicate or inhibit S.E. biofilms formation.Conclusion：①The experimental results observed Staphylococcus epidermidisbiofilms in vitro have strong pathogenic；②in New Zealand rabbits S.E. biofilms formation can be directly observed by scanning electronmicroscope and confirms for further research of cochlear implantationwhich provides the animal model and the support of experimental infection；③The clinical use of alternative antimicrobial agents such as H2O2,strong iodine can effectively eradicate or inhibit S.E. biofilmsformation.