| Objective:To detect the physicochemical properties and biological safety of TLM(Ti-3Zr-2Sn-3Mo-25Nb) alloy coated by micro-arc oxidation and micro-arc oxidationhydrothermal treatment. To assess the tissue-compatibility and material-tissueinteraTion by subcutaneous implantation experiment. Explore the possibility of itsclinical application as microimplant anchorage and provide experimental basis for itsclinical application in the future.Methods:1.The surface morphology, the phase, elemental composition, Wetting Angle androughness of the coatings of TLM alloy, which is treating by micro-arc oxidation andmicro-arc oxidation-hydrothermal treatment, were characterized by SEM, XPS, XRD,contact angle measuring instrument and surface roughness meter respectively.Statistical analysis was accomplished by one-way classification ANOVA and LSD-ttest (SPSS13.0).2.Twelve healthy adult New Zealand rabbits were involved in this study. TLM allyspecimens were treated by micro-arc oxidation、micro-arc oxidation-hydrothermaltreatment and no processing, and implanted into one side of the back subcutaneoustissue in rabbit respetively. The contrast materials(pure Ti) were implanted into theopposite side in the same animals. According to YYT0127.8-2001standard, thesamples were taken at1,4weeks after the implantation to observe fibrous capsulemorphology. Interfacial condition between test pieces and surrounding tissues wasobserved in light micrscope and SEM, and histological examinations were carried outthrough hematoxylin-eosin staining. Results:1.Observed by SEM, holes, multilayer overlap like hooks crater were distributedevenly on the two kind of surface of MAO grop and MAO-Ht grop. There werehydroxyapatite crytals evenly distributed aroud the hols of the MAO-Ht group, but theMAO group.2. The film was composed of Ti、Nb、Zr、Sn、Mo、O、Ca and P elements. And Ca/Pratio is1.64,1.54respeTively,close to the calcium and phosphorus ratio of the boneapatite.XRD indicated the phases in the film were mainly rutile phase and anatasephase TiO2. Hydroxyapatite phase Ca10(PO4)6O can be found in XRD diffration peaksof the MAO-Ht surface.3.The surface roughness of TLM surface by two methord of surface treatment weregreaterthan untreated. A significant difference was observed. But there was nosignificant difference between the MAO group and MAO-Ht group.(p<0.05)4.The wetting angles of TLM surface by two methord of surface treatment wereobviously less than untreated group. A significant difference was observed.Significant difference was observed between the MAO group and MAO-Htgroup(p<0.05).5.Vascularized capsules formed around specimens, and became thinner andtransparent along with the implantation time prolonged, the number of capillaries inthe capsules was also increased. Inflammatory reaction, with the time went, thenumber of inflammatory cells decreased. The inflammatory response of the twoexperimental group were similar to the control group on different time points.6.When the capsules were seperated from the two coating surface, there were thebrush shape phenomenon. With the time proloned, they were more closed, the brushshape phenomenon were more obvious. The surface treated by MAO-Ht combinedwith the capsules more closely. By SEM,the results were similar to the result in thelight microscope. Fibrous tissues were adhereing to the rough surfaces, after4weeksholes of the alloy surface were full of the capsules tissues. It is unable to distinguishthe orginal surface morphology,in the MAO-Ht group.Conclusions: 1.micro-arc oxidation and micro-arc oxidation-hydrothermal treatment can form arough、porous、calcium-rich film on TLM surface, enhance the surface energy androughness in the best range of implant requirements. In addition, there was a uniformlayer of HA on MAO-Ht film, prompting that it may promote bond tightly to bonetissues which significantly increase the bonding power between implants and bonetissues.2.TLM alloy in untreated，micro-arc oxidation and micro-arc oxidation-hydrothermaltreatment possesses good tissue-compatibility.3.The porous surface of TLM which treated by micro-arc oxidation and micro-arcoxidation-hydrothermal is good for the grow of soft tissue, and can increase thebonding power between implants and bone tissues.The existence of apatite crystallingphase effetively enhance the combination between the implant materials and fibrousconnective tissue, suggesting that the sealing between implant and soft tissue is better,so that prevent away from peri-implantitis to the microscrew-bone interface.Theexistence of apatite crystalling phase effectively enhance the combination between theimplant materials and fibrous connective tissue, suggesting that the sealing betweenimplant and soft tissue is better, so that prevent away from peri-implantitis to themicroscrew-bone interface. |
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