The Research On The Toxicity Effect And Molecular Mechanism Of Traffic Pollution To Male Reproductive System

Objective: This study established the model of exposure to atmosphericparticulate pollutants and scene of environmental pollution by traffic pollution toinvestigate the effect of pollutants on male mice’s reproductive system. This study canprovide scientific foundation for promoting procreative health and making availabilitymeasures.Methods: Establish exposure to the scene of environmental pollution by trafficpollution(30Kunming male mice were randomly divided into heavily polluted group,lightly polluted group and blank group, i.e.10in each group, and every mice wereexposed10h every day, exposed for35d) and Abdominal cavity exposure route (40clean ICR male mice were randomly divided into four groups respectively (PBScontrol group，low dose group(0.4mg/ml), middle dose group(2mg/ml) and high dosegroup(10mg/ml)). According to10ml/kg, we did one intraperitoneal injection per daylasting35d. Development of genital organs, sperm density，sperm abnormality，thepathological changes of testis and epididymis, damage of testis cells，the testosteronelevel in the blood and the relative expression level of P450scc and Star m RNAamong different groups were detected and analyzed after experiment.Results: Exposure to the scene of environmental pollution by traffic pollutionand abdominal cavity exposure route: Compared with the blank group, there isstatistical difference in body weight, testis weight, epididymal weights, and thedamage of testis cells in heavily polluted group(P＜0.05), but not in lightly pollutedgroup; there is statistical difference in sperm density, sperm activity and observe thepathological changes in both heavily polluted group and lightly polluted group(P＜0.05). Compared with the control group, there is statistical difference in epididymal weights and viscera coefficient of epididymis in high dose group (P＜0.05), there isstatistical difference in serum testosterone and the relative expression level ofP450scc mRNA in high dose group(P＜0.05), observed pathological changes of testisand epididymis in both middle and high dose group.Conclusion: Traffic pollution and main constituent of it-automobile exhaustsparticulate may do damage on male mice’s reproductive with the mechanism ofdamaging directly testis cells and affecting serum testosterone exudation and therelative expression level of P450scc mRNA.