| Objective：1. The expression of CCR7in Siha cells was detected after the cells were exposed todifferent X-ray irradiation doses，cisplatin and concurrent X–ray irradiation pluscisplatin in vitro，in order to analyze them to the CCR7expression influence.2. To study the relationship between CC7and radiotherapy of cervical squamous cellcarcinoma, examine the changes of CCR7expression in uterocervical tissues ofcervical carcinoma patients after the cervix exposed to different irradiation doses.Methods：1. Siha cells lines were irradiated with0Gy,2Gy,10Gy,20Gy and30Gy X-ray dosesin single fraction or received different concentrations of cisplatin (0μ g/ml,0.1μg/ml,1μ g/ml,2.5μ g/ml,5μ g/ml,10μ g/ml and20μ g/ml), respectively. Theexpression of CCR7was detected by western blot method after24and48hours.2. Siha cell lines received concurrent X–ray irradiation （2Gy） plus cisplatin, orsingle cisplatin of different concentrations (0μg/ml,0.1μg/ml,5μg/ml, and20μg/ml), respectively. Compare the expression levels of chemokine receptor CCR7between the two groups, after they were treated for48hours.3. The total of twelve cases which were diagnosed as cervical squamous cellcarcinoma at our institution was included in this study. They were divided intoIMRT group and RT plus BT group. Obtain patient’s informed consents as apremise of the study. Through the continuous X-ray irradiation or afterloadingirradiation in cavity, we would clip a few tumor tissue specimens by biopsyforceps when the patients were continuously irradiated to specific dose. The IMRTgroup cases were irradiated with6MV X-ray to0Gy,4Gy,12Gy,22Gy and30Gy;and the RT plus BT group cases were irradiated with with6MV X-ray and γ-rayto0Gy,4Gy,16Gy,34Gy,42Gy,57Gy. The specimens were preserved in liquidphase, and then chemokine receptor CCR7was detected by western blot method. Results：1. The expression of CCR7in2Gy，10Gy，and20Gy groups is higher than controlgroup，while the group of30Gy is lower than control group with statisticalsignificance when the Siha cell lines were irradiated after24hours（P＜0.05）.After48hours，the expression of CCR7in2Gy and10Gy groups compared tocontrol group reveals a significantly increase （P＜0.05）. No statisticallysignificant differences were found between the groups of20Gy and30Gy andcontrol group. The expression of CCR7in10Gy and20Gy groups decreasedsignificantly with the time prolongs from24hours to48hours（P＜0.05）.2. When Siha cell lines were treated respectively by cisplatin for24hours, theexpression of CCR7in1μg/ml and2.5μg/ml and10μg/ml groups had asignificantly increase compared to control group（P＜0.05）. While for48hours,the expression of CCR7in0.1μg/ml and5μg/ml and10μg/ml groups had asignificantly increase and the level of CCR7of20μg/ml group decreasedsignificantly compared to control group（P＜0.05）. The expression of CCR7in20μg/ml group decreased significantly with the time prolongs from24hours to48hours（P＜0.05）.3. At the concentration of20μg/ml Cisplatin plus2Gy, the concurrentchemoradiotherapy could reduce the CCR7expression in Siha cells comparedwith Cisplatin alone. While the concurrent chemoradiotherapy increased theexpression of CCR7compared to cisplatin alone at the concentration of0.1μg/mlCisplatin plus2Gy（P＜0.05）.4. IMRT group：the expression of CCR7in22Gy and30Gy group is lower thancontrol group with statistical significance. Though the expression of CCR7in4Gyand12Gy group is slightly higher than control group, differences are notsignificant among them. RT plus BT group：the expression of CCR7in16Gygroup is higher than control group, while the groups of43Gy and57Gy are lowerthan control group，respectively. The differences are all significant（P＜0.05）.Conclusion: 1. The CCR7expression in Siha cells can be increased by single low doses（2Gy，10Gy）of X-ray irradiation. However，the higher irradiation dose （30Gy）maynot downregulate the CCR7expression significantly in vitro.2. Low concentration of cisplatin can promote CCR7expression in Siha cells.However, high concentration of cisplatin can reduce CCR7expression.3. Concurrent high concentration of cisplatin plus X-ray irradiation can inhibit CCR7expression in Siha cells better than Cispltin alone group. And low concentration ofcisplatin plus X-ray irradiation can up regulate the level of CCR7expression.4. The CCR7expression in uterocervical tissues increased in the early stages ofcervical cancer treated by radiotherapy. However，CCR7expressio significantlyreduced in the late stage. |
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