| Background and objective:Hepatocellular carcinoma is the fifth most common cancer in the world.HCC has several interesting features, and one of the most important features isthe difference between men and woman.Globally, liver cirrhosis is the most frequent underlying cause of HCC. Andcirrhosis usually lead to high pressure of protal vein. When portal hypertension,blood cell would stay in spleen, then it will lead to hypersplenism. The researchof hypersplenism is lagging behind, no significant progress. The current study ofhypersplenism is mainly focused on how to deal with the pathological spleen.Retain it or remove it. We believe that splenectomy can slow the progressof cirrhosis of the liver and promote liver regeneration, thus reducing the risk ofhepatocarcinogenesis. But there still is not enough evidence to prove the view.Sex hormones is proved to have some relationship with hepatocarcinogenesis.But we don’t know which hormone and how it works. This study is mainlyaimed for the relationship of hypersplenism, gender facor andhepatocarcinogenesis. So that, the result of this study would to provide the evidence for the treatment of patient with liver cirrhosis and HCC in clinical.Methods:Firstly, we repeat to establish the model of portal hypertension withhypersplenism by pre-experiments in SD rats. Secondly, inducing the HCC ofthe SD rats by Diethylnitrosamine. Thirdly, do splenectomy to some SD rats indifferent times and orchiectomy to the other ones. After14weeks we observethe process of carcinogenesis and the different degree of liver fibrosis indifferent groups.Results:1. Hypersplenism rats liver fibrosisi and the incidence of the tumor was higherthan the conrol group(P<0.05); Compared to hypersplenism group, earlysplenectomy group (4weeks) rats had a significantly lower rate ofcancer(P<0.05). And after the splenectomy, the erythrocyte and platelet countswas higher than that of the hypersplenism group. While the difference ofhepatocarcinogenesis in the female group was not that obvious.2. Late splenectomy group (9weeks) rats showed good effort of promte theerythrocyte and platelet counts in the blood. But there was no significantdifference of the incidence of the tumor between splenectomy group and thehypersplenism group.3. Androgen deprivation groups rats had a higher incidence of HCC anddegree of liver cirrhosis than hypersplenism group (P <0.05). Compare to thecontrol group, there was no significant difference on that. And at any level, theresult of androgen deprivation groups rats was more likely that of femalehypersplenism group.Conclusion:1.Among male rats, splenectomy can delay the development of liver cirrhosis,and have good effort to hepatocarcinogenesis. But in female rats, removethe pathological spleen may not show that protection. 2.In male rats, when the degree of liver fibrosis is extremely high, splenectomyis only beneficial to improve the number of blood platelets and erythrocyte, butshow little protective effort of liver.3.When hypersplenism, androgen deprivation can marked reduce HCCincidence,and the development of liver cirrhosis will be slow down. That’smore likely to a female hypersplenism one shows. So, androgen may do harm tothe liver. |
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