Study On The Antiproliferation Effect Of Targeting Of Survivin SiRNA Transfection Combined With5-FU On Liver Cell Line HepG2

Objective:To investigate the anti-proliferation effect of (small interference RNA) siRNA against survivin combined with (fluorouracil)5-FU on human liver cancer cell line HepG2.Methods:HepG2cells cultures were divided into five groups:blank control group, negative control group,5-FUgroup, siRNA-survivin group,siRNA-survivin+5-FU group. Lipofectamine TM2000was used to transfectHepG2cell. The expression of survivin mRNA was detected by RT-PCR. The effects on cell proliferation and apoptosis were analyzed by MTT assay and flow cytometry respectively.Results:The experiment of RT-PCR showed that the expression of survivin mRNA in siRNA-survivin group and siRNA-survivin+5-FU group were lower than that in other groups(p<0.05), but its levels in blank control group, negative control group and5-FUgroup had no significant change (p>0.05). The MTT assay indicated that the antiproliferation rate of siRNA-survivin+5-FU group was51.38%±1.35. The antiproliferation rate of this group was higher than that in any other group(p<0.05). Flow cytometry was indicated that the apoptosis rate of siRNA-survivin+5-FU group was higher than any other group(p<0.05).Conclusion:siRNA against survivin combined with5-FU can specifically suppress its expression,inhibit the proliferation of HepG2and induce apoptosis in common.