A Study Of The Expression Of Special AT-rich Sequence Binding Protein1(Satb1) In Oral Tumors

Objectives:1. To detect and explore the different expression of SATB1protein in oral benign and malignant tumors and normal gingival epithelium.2. To detect the expressions of SATB1mRNA and protein in primary tumour, metastatic lymph node lesions and pericancerous tissue of human oral squamous cell carcinoma(OSCC), and analyze the relationship between SATB1expression and the occurrence, progression, invasion and lymphatic metastasis of OSCC.3. To explore the expressions of SATB1mRNA and protein in primary tumour of adenoid cystic carcinoma (ACC), and analyze the relationship between SATB1expression and ACC occurrence and progression.Methods:1. Sixty-six cases of oral malignant tumors paraffin-embedded samples, which included52cases of OSCC,14cases of ACC,4cases of pleomorphic adenoma,4cases of leukoplakia, and2cases of normal oral mucosa as the control group, were detected by immunohistochemical staining. The expression of SATB1protein was qualitatively analyzed.2. Fifty-two cases of OSCC paraffin-embedded samples, which included36cases without metastasis,16cases with metastasis,16cases with metastasis of lymph node, and12cases of pericancerous tissues as the control group, were detected by immunohistochemical staining, and the expression of SATB1protein was qualitatively analyzed.3. Fifty-two cases of OSCC fresh samples, which included36cases of primary tumour without metastasis,16cases of primary tumour with lymphatic metastasis, which included6cases of metastasis lymph nodes, and12cases of pericancerous tissue of surgical resection in the same period as the control group, were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression level of SATB1mRNA was semi-quantitative analyzed.Results:1. The expressions of SATB1protein in36/52cases (69.2%) OSCC and14/14cases (100%) ACC were obviously higher than those in benign tumors and in normal gingival epithelium. Weak positive expressions of SATB1protein were found in1case in pleomorphic adenoma and in leukoplakia respectively. There was no obvious expression in normal oral mucosa. Immunohistochemical analysis showed that the expression of SATB1was located in nucleus and cytoplasm of OSCC, and in nucleus of ACC.2. The expression of SATB1protein in52cases of OSCC was obviously higher than that in12cases of pericancerous tissue (P<0.01). The expression of SATB1protein in histology grade Ⅱ～Ⅲ OSCC was obviously higher than that in histology grade Ⅰ (P<0.05), and it was obviously higher in clinical stage Ⅲ～Ⅳ OSCC than that in histology grade Ⅰ～Ⅱ (P<0.05). However, there was no correlation between the expression of SATB1and the age, gender, primary site and size of tumor.3. The expression of SATB1protein in16cases of primary tumour with lymphatic metastasis was higher than that in36cases without metastasis (P<0.05). Besides, there was no significant statistic difference between the expression of SATB1in16cases of metastatic lymph nodes and that in16cases of primary site of OSCC (P>0.05).4. The expression of SATB1mRNA in52OSCC fresh samples was significantly higher than in12cases of pericancerous tissue (P<0.01). It was higher in16with metastasis than in36cases without metastasis (P<0.05). And in6metastatic tissue in lymph nodes, the expression of SATB1mRNA was higher than in16cases of primary lesions in tumor(P<0.05).5. The expression of SATB1protein was positively associated with that of SATB1mRNA in OSCC (r=0.808, P<0.01).6. SATB1protein and SATB1mRNA in all cases of ACC showed strong positive expression. The two expression has a positive correlation(r=0.877, P<0.01).Conclusions:1. The expressions of SATB1protein and SATBlmRNA in oral malignant tumors are significantly higher than those in oral benign tumors and in normal gingival epithelium, which suggests that SATB1may play a important role in the occurrence and development of oral malignant tumors.2. The higher expressions of SATB1protein and mRNA in OSCC suggests that the expression upregulation of SATB1protein and SATB1mRNA may have a positive relation with the malignant degree, invasion, metastasis of OSCC, and indicate that the changes of SATB1expression level may help to judge the metastasis and the prognosis of OSCC.3. The expressions of SATB1protein and SATB1mRNA in primary tumor of metastasis lymph node group are higher than that no metastasis group in OSCC, and lower than that in lymphatic metastasis tissue. It indicates that SATB1may be one of the promoting genes to OSCC lymphatic metastasis.4. There are strong positive expressions of SATB1protein and SATB1mRNA in ACC, indicating that SATB1may play a significant role in the occurrence and progression of ACC.