| Objective To explore the relationship between gene P21and P53expression and single nucleotide polymorphism(SNP) and the hepatocellular carcinoma (HCC)Methods The research was designed as a case-control study based on hospital. Reverse Transcription Polymerase Chain Reaction (RT-PCR) was used to measure the expression level of P21and P53in100cases of HCC and100cases of not-tumor patient, Genotypes of P213’UTR C/T and P53intron6G/A were detected using polymerase chain reaction-restriction fragment length polymorphism. All the statistical analyses were performed with SSPS software (version13.0). Student’s t-test was used to compare mean value of the samples, while categorical data were compared with the use of the chi-square test or the Fisher’s exact probability. Hardy-Weinberg genetic equilibrium (HWE) for all two SNPs was tested in controls using SHEsis software. Odds ratio (OR) and95%confidence interval (CI) were obtained from unconditional univariate and multivariable logistic regression model to evaluate associations between all two SNPs and HCC risk. All tests were two-sided with a is0.05.Results(1) P21mRNA expression level was higher in the hepatocellular carcinoma patients than in the not tumor patient (P<0.05). In age, sex, smoking, drinking, HBsAg patient and not-HBsAg defining cancer, presence of liver cancer family history, AFP there were no statistically significant differences between the groups with further performed analysis (P>0.05).(2) P53mRNA expression level was higher in the hepatocellular carcinoma patients than in the not tumor patient (P<0.05). In age, sex, smoking, HBsAg patient and not-HBsAg defining cancer, presence of liver cancer family history, AFP there were no statistically significant differences between the groups with further performed analysis (P>0.05). However, P53mRNA expression level was higher in the ever alcohol users than in the non-drinkers, and these differences were statistically significant (P<0.01)(3) Further study showed no correlation in P21mRNA and P53mRNA expression level in the case group (P>0.05).(4)Association of P213’UTR C/T SNP with the risk of HCC The genotype frequency distributions of P213’UTR C/T were in agreement with the HWE among the controls (P>0.05). The genotype and allele frequencies of the polymorphisms were not statistically different between cases and controls (P>0.05). No significant association was observed between the C/T genotype and the T/T genotype of P213’UTR C/T compared with the C/C genotype (adjusted OR=0.90,95%CI:0.31-2.65, P=0.85; adjusted OR=0.35,95%CI:0.04-2.79, P=0.32, respectively). No significant association was observed with T allele in the risk of HCC (adjusted OR=0.79,95%CI:0.27-2.28, P=0.67). Stratification analysis showed that after stratified with age, in subgroup of age>60, HCC risk of group of A allele were7.54times that of C allele (P=0.047).(5)Association of P53intron6G/A SNP with the risk of HCC The genotype frequency distributions of P53intron6G/A were in agreement with the HWE among the controls (P>0.05). The genotype and allele frequencies of the polymorphisms were not statistically different between the cases and the controls (P>0.05). No significant association was observed between the G/A genotype and the A/A genotype of P53intron6G/A compared with the G/G genotype (adjusted OR=1.42,95%CI:0.48-4.22, P=0.53; adjusted OR=1.13,95%CI:0.12-6.77, P=0.90, respectively). No significant association was observed with A allele in the risk of HCC (adjusted OR=1.35,95%CI:0.49-3.71, P=0.57). After stratified with age, sex, smoking and drinking, no risk increasing genotype was found. (6)The gene interaction of P213’UTR and P53intron6didn’t exist in the etiology of HCC.Conclusions(1)P21gene and P53gene maybe take part in the progress of HCC.(2)Drinking may increase the level of expression of P53mRNA in hepatocellular carcinoma patients.(3) P213’UTR C/T C/T+T/T genotypes may increase the risk of HCC among aged>60years subjects. |
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