A Study Of Molecular Genetics On The Expression Of DLC-1 Gene And Gene Mutation In Human Primary Hepatocellular Carcinoma

Objective To investigate the function of DLC-1 gene in the pathogenesis of primary hepatocellular carcinoma (PHC) and the molecular mechanism of PHC carcinogenesis. Methods Semi-quantitive reverse transcriptase-polymerase chain reaction(RT-PCR) and single strand conformation polymorphism(SSCP) were used to detect the expression variation and gene mutation of DLC-1 gene in 30 PHC tissues, their corresponding adjacent liver tissues and normal liver tissues of 20 non-liver disease patients. Results (1)The expression rate of DLC-1 gene was 76.67% (23/30) in the PHC tissues , while the expression rate of DLC-1 gene was 100% in the corresponding adjacent tissues (30/30) and in the normal liver tissues(20/20). The expression rate of DLC-1 gene in the PHC tissues was significantly lower than that of the adjacent tissues (P<0.01) and normal liver tissues (P<0. 05); (2)The expression level of DLC-1 gene in the PHC tissues, their adjacent tissues and normal liver tissues were 0. 351 ±0. 0034( x ±SE) , 0. 598 ± 0. 0079( x ± SE) and 0. 814 ± 0. 137(x ±SE) respectively. The expression level of DLC-1 gene in the PHC tissues was significantly lower than that of the adjacent tissues (P<0. 05) and normal liver tissues (P<0. 01);there was no significant difference between adjacent liver tissues and in normal liver tissues (P>0. 05) ;(3) The expression level of DLC-1 gene in the low-differentiated PHC tissues was much lower compared with the high and middle differentiated PHC tissues (P<0.01); (4) There were significant differences in the expression level of DLC-1 gene between the PHC tissues with satellite focuses transferred by PHC and that of control group (P< 0.05); (5) The expression quantity of DLC-1 gene in PHC tissues was not closely related to PHC patients age and gender(P>0.05); (6) None of the DLC-1 gene mutation was found in all the hepatocellular carcinoma samples. Conclusion (1)The positive expression rate of DLC-1 in the PHC tissues...