| Endometrial carcinoma is one of the three malignant tumors of female reproductive system, accounting for7%of malignant tumors of female and20%-30%of malignant tumors of female reproductive system. In recent years, the incidence of Endometrial carcinoma showed a upward trend in worldwide. The development of endometrial cancer is a multi-factor, multi-step, multi-stage and complex process of multiple gene variants, involving multiple aspects of cell proliferation, apoptosis and angiogenesis, and so on. budding uninhibited by benzimidazoles-1(Bub1)and mitotic arrest deficient-2(Mad2) are Important component of spindle checkpoint, spindle checkpoint is an evolutionarily highly conserved mitosis monitoring system to ensure the fidelity of chromosome during mitosis.If the expression of Bub1and Mad2are aberrantly would have caused the function of spindle checkpoint defected,then lead to chromosomal instability and aneuploid. p53is a tumor suppressor gene, it product into two subtypes:the wild type and mutanted type. The function of wild type p53was to inhibit tumorigenesis; mutant type of p53protein (Mtp53) have lost the function of inhibit tumorigenesis,and it was unable to control cell proliferation. To investigate the expression of the regulatory proteins of the mitotic process in cell cycle,may discover the pathogenesis of tumors, in order to give early diagnosis and treatment of the tumor, may also have the value to evaluate the prognosis of patiens. In this study, the expression of Bub1, Mad2and Mtp53proteins were detected by immunohistochemical method in each group of endometrial, endometrial hyperplasia, complex endometrial hyperplasia (30cases in each group) and endometrial carcinoma (63cases) and investigate the correlation among them. ObjectiveTo investigate the expression of Bub1, Mad2and Mtp53proteins in in each group of endometrial, endometrial hyperplasia, complex endometrial hyperplasia and endometrial carcinoma and the correlation among them.Methods1Study ObjectFrom January2007-January2011,63cases surgical removal of archived paraffin specimens has been confirmed for endometrial carcinoma in The Third Affiliated Hospital of Zhengzhou University, Department of Pathology.And to take paraffin specimens of endometrial, endometrial hyperplasia, complex endometrial hyperplasia (30cases in each group)by endometrial curettage diagnosed during the same period.All patients had not received any hormone therapy, radiotherapy or chemotherapy.2MethodsThe expression of Bub1, Mad2and Mtp53proteins were detected by immunohistochemical SP method in each group of endometrial, endometrial hyperplasia, complex endometrial hyperplasia and endometrial carcinoma.3Statistical method:SPSS13.0software was used for statistical analysis of data, Comparison between different groups used χ2Test, Spearman correlation analysis was used for investigate the correlation of the the three factors."α=0.05" was set to standards of statistical analysis. Results1Patients’age in four groupsThe age of patients in group endometrial, endometrial hyperplasia, complex endometrial hyperplasia, endometrial carcinoma were(49.43±3.50)years,(49.27±5.43) years,(49.13±5.47)years,(50.77±4.82)years.There was no statistically significance among them (P>0.05).2The expression of Bubl protein in four groupsThe positive rate of Bubl in endometrial, endometrial hyperplasia, complex endometrial hyperplasia, endometrial carcinoma were86.67%,76.67%,56.67%,28.57%.The positive rate of Bubl protein was decreased with the severity of endometrial tissue lesions. The expression of Bub1was negatively related to endometrial lesions(ra=-0.501±P<0.05).There was a statistically significant difference between clinical stage I and clinical stage II(P<0.05). The expression of Bub1showed a statistically significant difference between Well-differentiated and Moderate degree of differentiation of endometrial carcinoma(P<0.05). Found no significant correlation with lymph node metastasis(P<0.05).3The expression of Mad2protein in four groupsThe positive rate of Mad2in four groups were23.33%,36.67%,56.67%,85.71%. The positive rate of Mad2protein was increased with the severity of endometrial lesions. The expression of Mad2was positively related to endometrial lesions(rb=0.514, P<0.05).There was a statistically significant difference between clinical stage I and clinical stage II(P<0.05). The expression of Mad2showed a statistically significant difference between Well-differentiated and Moderate-differentiation of endometrial carcinoma (P<0.05). Found no significant correlation with lymph node metastasis(P<0.05). 4The expression of Mtp53protein in four groupsThe positive rate of Mtp53in four groups were0,0,26.67%,69.84%, The positive rate of Mtp53protein was increased with the severity of endometrial lesions. The expression of Mad2was positively related to endometrial lesions(rc=0.635, P<0.05).There was a statistically significant difference between clinical stage I and clinical stage Ⅱ(P<0.05). The expression of Mtp53showed a statistically significant difference between Well-differentiated and Moderate-differentiation of endometrial carcinoma (P＜0.05). Mtp53was related to lymph node metastasis (P<0.05).5The correlation among Bubl, Mad2and Mtp53proteinsIn endometrial carcinoma group, there were both positive expression of Bubl and Mad2proteins were11cases,2cases were both negative expression, The expression of Bubl with Mad2was negatively correlated (r1=-0.636, P<0.001); There are11positive cases of Bub1wiht Mtp53positively expression in endometrial carcinoma group,12cases were both negatively expression of them, a negative correlation was found between Bub1and Mtp53proteins (r2=-0.325, P<0.05).There were both positive expression of Mad2and Mtp53proteins were41cases,6cases were both negative expression. The expression of Mad2with Mtp53was positively correlated (r3=0.484, P＜0.001).ConclusionThe aberrantly expression of Bubl, Mad2and Mtp53in endometrial carcinoma tissue may be involved in the development process of endometrial carcinoma. |
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