Expression And Significance Of ERβ In Bladder Urothelial Carcinoma

ObjectiveTo study the expression and significance of ERP in bladder urothelial carcinoma and to evaluate their significance in the generation, progression and prognosis of the bladder cancer. To prove whether the gender differences in the incidence of bladder cance related to estrogen receptor and to understand the situation of ERβ as a targetfor endocrinetherapy of bladder urothelial carcinoma.MethodsThe expression of ERβ in64cases of human bladder urothelial carcinoma tissue,64cases of adjacent bladder cancer and25normal bladder tissue were detected by Immunohistochemistry S-P and Reverse transcription polymerase chain reaction (RT-PCR), respectively.The correlation was analysed statistically, between the expression of ERβ protein and mRNAand clinicopathological parameters of human bladder urothelial carcinoma.Using SPSS package for windows17.0statistical analysis to test all data.The expression of ERP in two gourps is compared by x2test; Different binary comparison between the groups uses the Wilcoxon analysis.The clinical data, clinical stage and histological grading between groups is tested by Pearson correlation analysis and the level for significance tests is0.05. ResultsThe positive rate of ERP protein and mRNA in malignant specimens was59.3%and0.271±0.022, while that in the adjacent bladder cancer tissue was21.8%and0.112±0.013and that rate in the nonnal bladder tissue respective is8.0%and0.06±0.008.The expression of ERβ protein and mRNA was significantly different with the adjacent bladder cancer tissue and nonnal bladder tissue (P<0.05). low-level and high-levelThe further statistical analysis showed that the ERβ protein and mRNA expression was correlated to the lymph node metastasis and the relapse of bladder urothelial carcinoma (P＜0.05), but had no correlation to the sex of TBCC (P>0.05).Lymph node metastasis and recurrence was significantly higher than in patients without lymph node metastasis group and the initial issuance of patients, and there were significant differences (P<0.05).The positive rates of ERβ protein and ERβmRNA in various pathological type and clinical stage were as follows:48.5%、0.198±0.011of low-level,68.9%、0.274±0.042of high-level.Known from the number, the ERβ mRNA and protein expression of high-level were significantly higher than the that of low-level group (P＜0.05). The positive rates of ERβprotein and ERmRNA in various pathological type and clinical stage were as follows:34.2%、0.231±0.041of Ta-T1and61.5%、0.3±0.033of T2-T4.There was significant difference of the positive rate and expression intensity between Ta-T1and T2-T4(P＜0.05). E R β m R N A and ERβ protein correlation analysis showed that both positively correlated (P<0.05)ConclusionThe occurrence about the gender differences of bladder urothelial carcinoma is Unrelated to the estrogen and its receptor. We can indicate from Lymph node metastasis and recurrence of group expression that, ERβ is related to the tumor invasiveness and prognosis. With the pathological grade andclinical stage increased, the expression of ERβ in the gene and protein levels gradually increased.The expression of ERβ of Invasive (T2-T4) was significantly higher than the superficial (Ta-T1),having asignificant difference (P<0.05). The increases of ERβ protein depends on the increases can be as one detection index of diagnosis,treatment, condition judgment and prognosis.