Extract Ginkgo Biloba Induced U251Glioma Cell Apoptosis And Its Mechanism Of Research

Objected:To explore the anti-tumor effects of extract ginkgo biloba including the possible mechanisms involved in the suppression of the human glioma cell line U251progression.Method:After U251were treated with various concentrations of extract ginkgo biloba (60、100、150、200、250mg/L), CCK-8was performed to examine the effect of extract ginkgo biloba on the proliferation of glioma cells. Hoechst33342fluorescent dye detection cell apoptosis form, Annexin V/PI staining assay was used for investigating the ability of the extract ginkgo biloba to induceU251cells apoptosis. fimmunohistochemical method to detect BCL-2expression. Statistic package of SPSS13.0was used for the data analysis and significant differences between means were evaluated by ANOVAanalysis.Result:1. CCK8assay shows extract ginkgo biloba (100,150,200,250mg/L) role12,24and48h all can inhibit U251cell proliferation. With the role concentration increases or function is the extension of time inhibition rate also increase, compared with controls are statistically significant difference (P<0.01), the difference between the two is also a statistically significant (P<0.05), but60mg/L extract ginkgo biloba12,24,48h to U251role there was no significant effect on cell proliferation, compared with the control was statistically significant (P<005).ginkgo biloba extract treatment group were visible nuclear enrichment density of strong fluorescence mass, nuclear chipped and apoptotic body and other typical of the cell apoptosis features, and with the increase of the drug concentration, apoptosis morphological changes the more visible, and the negative control group and solvent group cell size uniform, into the blue uniform dispersion fluorescence;3. Extract ginkgo biloba100,150,200,250mg/L apoptosis rate compared with controls are statistically significant difference (P<001). Has a certain dose dependent relationship,60mg/L role apoptosis rate after no significant effect.4. Different concentration extract ginkgo biloba role after24h, the SP dyeing of microscope and, in gliomas apoptosis BCL-2of genes in tumor cells of the main expression in the cytoplasm, the positive expression in the cytoplasm is appeared in brown or yellowish-brown dyeing, the darker the BCL-2that the amount of expression, the more with the increasing of the concentration of extract ginkgo biloba, cell cytoplasm tan in dyeing expression more and more shallow, explain BCL-2in the expression of cell plasma inside gradually reduce, prompting the extract ginkgo biloba can change U251cell apoptosis of gene protein expression.