Expeirmental Study Of Hyperbaric Oxygen On The Costal Catrilage Membrane Into The Bone Mass

Background: The frequently used in orthopedic clinical autologous rib cartilage, earcartilage, nasal cartilage as a surgical materials, transplanted cartilage auricularreconstruction surgery, rhinoplasty, cartilage defect. But when more of the costal cartilagedosage may cause a certain degree of thoracic development and morphology for the Districtpostoperative thoracic deformity. Poor ability to regenerate cartilage defects with autologouscartilage available for and limited organization, the cartilage graft is bound to cause thedamage and the appearance of the donor site function abnormalities or deformities. Order toreduce the thoracic deformity of incidence, only the additional sufficient amount of cartilagetissue in order will be for the damage reduced to a minimum, some scholars at home andabroad through a number of animal experiments to prove the ability of the cartilageregeneration of cartilage, while the high-pressure oxygen (HBO) can accelerate fracturehealing and bone formationThe purpose of the experiment: cut under study HBO auxiliary autologous costalcartilage costal cartilage membrane rib cartilage repair and regeneration, as well as intocartilage volume change in the HBO role.Methods:8adult rabbits, weight1.8-2.5kg, were randomly divided into two groups,each group of four experimental groups, intact cartilage membrane and rib cartilage link atthe same time to remove a section of rib cartilage defect site by direct suture, cartilage debrisafter replantation replanted suture and cartilage debris plus fibrin glue to fill the gap after theclosure of three kinds of methods of treatment, three kinds of methods in each experimentalgroup rabbits on both sides of rib cartilage paired after1d into the hyperbaric oxygen (HBO)treatment of cabin pressure0.2MPa pressure15min, continuous regulator oxygen60mindecompression15min,1/d2weeks for a course, a total of four courses for eight weeks; Thecontrol group rabbits to deal with in the same way. The two groups of rabbits were sacrificedat different postoperative time period to observe the gross morphology of the chest andrepair section of HE staining biopsy.Results: the experimental group operation in rabbits after healing well, no infection,death. Hyperbaric oxygen group overall weight is slightly lower than that of the controlgroup in rabbits. Thoracic overall shape is good, no obvious collapse, deformation and other deformities, hyperbaric oxygen group generation of cartilage and smooth surface, texturehard, cartilage membrane can be whole again stripped, the processing method of no obviousdifference between film, compared with preoperative slightly thickened, the conventionalgroup formed part of intact cartilage, formed part of cartilage is incomplete, part slightlyangulated, cartilage film can be stripped. In4,8,12,16of different time periods, respectively,in the HBO group and control group randomly selects the conventional two experimentalrabbit, go after death, along the original operation incision will hare sternal exposure, eachleft and right sides of each of the three root excision costal cartilage after closure of theintact cartilage membrane, a total of12root. Using the chi-square test method, tested in fourtime periods of costal cartilage membrane of the costal cartilage formation rate, can beobtained in the four groups of data in P <0.05, with statistical significance. Organizationalstrength testing, analysis after hyperbaric oxygen group to regenerate cartilage tissuebiomechanical strength and conventional control group there were significant differences.Conclusion:1, rabbits can be used as a simulation of people cut rib cartilage injuryafter thoracic stable animal model.2, back to the cartilage debris to promote the regenerationof hyaline cartilage repair depends on the diameter of the cartilage debris, close to its normalrib cartilage, is difficult to distinguish; hyperbaric oxygen can accelerate wound healingability, and can replace antibiotics to prevent infection occurred, the participation ofhyperbaric oxygen damage to the cartilage perichondrium heal quickly and completelyclosed the gap, to release the ability to better regeneration of cartilage, thereby reducing thethoracic deformity incidence, more fully available for clinical resources.