| Objective To investigate the alcohol behavior pattern and the genetic polymorphism of ADH3and ALDH2among Qinghai male Tibetan.Methods (1)Data concerning alcohol behavior was obtained by questionnaire. The survey is planed to arranged to the physical examination people healthy testing population in Centers for Disease Control and Prevention and Traditional Tibetan Hospital in QingHai province and procedures are followings:firstly, pick out male tibetan from all people who do their physical test, and then sample one patient every one; The survey is also planed to arranged to students in medical college of QingHai university, all of the boys in the class, which should be sampled in the way of cluster sample, should be investigated, excluding those who don’t want take part in the survey; So does the Han nationalism. All of those objects should be willing to participate in this survey. This survey include758persons from three sample sites, ages ranges of39.7±14.6,640files for survey analysis excluding26persons whose personal data is imperfect,430male for tibetan and302for han.(2) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the genotype of ADH3and ALDH2.Results (1)The alcohol drinking rate in Tibetan and Han are66.47%and69.54%respectively; and the safe drinking rate in Tibetan and Han are27.67%and28,16%;the hazardous and harmful drinking are20.21%and28.57%respectively,67.94%liquor is low-alcohol in Tibetan and64.28%for Han. The mainly drinking way are drinking together with dining in Tibetan and Han,and the ratio are79.44%and58.09%.respectively,the drinking together without dining is second.and12.89%and19.05%respectively. There is significant in drinking way(p<0.01), and so did reason of drinking, Most of the drinkers, both Tibetan and Han believe the drinking is the way to make friends, and the ratio are57.49%and51.34%respectively, then consider it is a kind of enjoyment, and27.87%and19.05%respectively. Logistics regression showed the blushing is protective factor, smoking and participate are risk factors to drinking.(2)The allele frequency of ADH3*2among Tibetan(0.08) is significantly lower than that for Han(0.14), while The allele frequency of ALDH2*2among Tibetan(0.22) is significantly higher than that for Han(0.18).The combination genotype is50%with normal ADH3and ALDH2, the normal ADH3mutant ALDH2is35.11%, mutant ADH3normal ALDH2is10.46%, and then mutant ADH3and ALDH2is4.41%.(3) ADH3and ALDH2are associated with drinking behavior in Tibetan male population, and especially with ALDH2.Conclusions The alcohol rate in male Tibetan is high and the drinkers are younger, there is unsafe behavior in the drinkers. The drinking behavior in Tibetan male were influenced by the genotype of ADH3and ALDH2, especially the genotype of ALDH2.compare to the Han, the frequencies of allele ADH3*1and ALDH2*2in Tibetan male are much higher than Han. It means there are more uncomfortable feeling after drinking in Tibetan than that of Han. The main genotypes are normal ADH3and ALDH2in Tibetan, and the population are higher risk to be alcoholic compare with the other genotype if they are drinking. And there are35.11%population with nomal ADH3and mutant ALDH2genotype which will be more sensitive to drinking alcohol. Health workers pay more attention to the risk drinkers, if they are dangerous drinkers or harmful drinkers and not developed to be the alcoholism, the intervention should be take immediately, it can effectively reduce the alcohol consumption and the alcohol-related problems. |
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