| Objective GOS is a prebiotic, it has many research about the impact of prebiotics on the intestinal mucosal barrier and the gut microflora mechanism. It has achieved good results in animal experiments. First, it can promote gut microflora balance and the growth of beneficial bacteria; second, it does not absorb, digest features, thus avoiding the side effects on the liver, kendey and other organs. Its special and complex causes altitude sickness, have a certain influence on the human body systems function, which includes the device quality, function, acute, chronic and other series of pathophysiological changes. Highatitude environment can cause the destruction of the intestinal barrier, resulting in translocation of intestinal bacteria and their toxins invasion, stimulate or aggravate the inflammation of various tissues and organs of the body, and reinforce each other, reinforce each other to form a "waterfall-like" reaction, eventually leading to the inflammatory response serious disorders, and immune function in the body to produce more damage, known as "mixed antagonistic response syndrome (mixed antagonist response syndrome. MARS) in the hypoxic environment of the plateau, galactooligosaccharides can be able to perform its protective effect on intestinal mucosal barrier, but both at home and abroad have not been studied, because the radical plateau, the possibility of using prebiotics to protect the intestine is particularly important, therefore, the subject of galactooligosaccharides radical plateau the rat intestinal flora changes in molecular biological experiments, and thus provide a theoretical basis for the gut microflora imbalance in the complex environment of the radical plateau and prevention.Methods Healthy Wistar rats of90animal experimental center of (Lanzhou University), weight (200±20) g, half male and half female, were randomly divided into three groups, were randomly divided into three groups, the plain control group (group A)304000m in the intervention group (group B)30304000m galactooligosaccharides in the intervention group (group C). By off-road vehicles radical Maxianshan (3848m) caused by hypoxia model, to the A, B, C, three groups of perfusion saline gavage needle or oligomeric galactose solution as follows:A, B, treated with normal saline1ml/kg,1/d, C, give galactooligosaccharides1g/kg/d, respectively,2,4,6d drawn, each time in each group were taken10. then, Use of quantitative PCR technique detailed, accurate quantitative changes to the high altitude hypoxia in rat intestinal flora to clear bacteria (Bifidobacterium), and harmful bacteria (such as E. coli) in the intestinal flora changes.Results E. coli fluorescence quantitative analysis as follows:A group of rat intestinal E. coli for the first2,4,6d, respectively (6.91±0.31)(6.92±0.33)(6.92±0.30); rat intestinal E. coli B,2,4,6d, respectively (7.25±0.36)(7.30±0.35)(7.37±0.33), corresponding to group A time compared were statistically significant (P<0.05). GOS after the intervention, group C rat intestinal E. coli’s2,4,6d, respectively (6.80±0.28),(6.84±0.38)(6.86±0.32) compared with group B corresponds to the time difference was statistically significance (P<0.05). Group B, and C in rat intestinal Escherichia coli with time and decreasing, but the difference was not statistically significant (P>0.05).Bifidobacterium fluorescence quantitative analysis as follows:Group A rats bifidobacteria’s2,4,6d, respectively (10.30±0.36)(10.30±0.39)(10.31±0.33); Group B rats bifidobacteria’s2,4,6d (9.89±0.41)(9.82±0.39)(9.73±0.44), with group A, the difference was statistically significant (P<0.05). GOS intervention, group C rats bifidobacteria2,4,6d, respectively (10.15±0.32)(10.11±0.34)(10.08±0.39) compared with group B the difference was statistically significant (P<0.05); B, and C rats bifidobacteria decreases with time but the difference was not statistically significant (P>0.05).After exposed to high attitude of hypoxia, The intestinal flora imbalance were significantly higher (P<0.05). After the intervention by galactooligosaccharides, the intestinal flora imbalance were lower (P<0.05).Conclusion Exposure to high altitude can effect the imbalance intestinal flora. Galactooligosaccharides can play an important role in regulating the intestinal flora imbalance. |
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