| Objective: To discuss the relationship between XPD gene polymorphism and the genetic susceptibility ofgastric cancer, liver cancer and colorectal cancer through examining the Lys751Gln gene polymorphism ofXPD, and analyze whether the risks of these cancers are increased when the interaction of this genepolymorphism and smoking and drinking happens.Methods: This study was carried out in Zhengzhou and Kaifeng city, Henan province of China, bycollecting a total of98patients with gastric cancers,76patients with liver cancers, and95patients withcolorectal cancers and80controls who were not with cancers. They all volunteered in this study. Wecollected their blood samples, detected genetic polymorphisms of XPDLys751Gln of those patients withdifferent cancers, using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP),and then analyzed the relationship between different genotypes and the genetic susceptibility of neoplasmin digestive tract. T test and statistical analysis by SPSS17.0were used to compare the differences of thecase group and control group in age distribution.χ2-test were used to compare the differences in sexdistribution between the case group and the control group. The Hardy-Weinberg Law was used to detect thedistribution differences of genotypes between groups and allele frequency. Unconditional Logisticregression technique was used after adjusting for confounders to calculate the ratio (OR value) and95%confidence internals, and analyze genetic polymorphisms of XPDLys751Gln and the genetic susceptibilityof gastric cancer, liver cancer and colorectal cancer. The statistics tests were two-sided probability tests andP <0.05indicates that the differences had statistically significances.Results: age distribution between patients with these cancers and controls had no statistical differences(P=0.861, P=0.103, P=0.052). The Hardy-Weinberg Law detection of control group indicated that there wasno statistical significant (P=0.102), which meant the control group selected by this study hadrepresentativeness of population.The distribution frequencies of Lys751Gln genotype in gastric cancer group, liver cancer group and colorectal cancer group were32.65%,34.21%and35.26%respectively, which was higher than that of thecontrol group (25%), and all these differences had statistical significances (P=0.038, P=0.002, P=0.016).The risk of having gastric cancer, liver cancer and colorectal cancer of individuals who carried Lys751Glngenotype increased1.486times (P=0.025),2.127times (P=0.016) and1.212times (P=0.007) respectively.Lys751Gln genotype was dangerous genotype of suffering attacks from gastric cancer, liver cancer andcolorectal cancer.The concurrence of carrying751Gln allele and smoking didn’t have interactions in the process of gastriccancer and colorectal cancer (S≈1), and the risk wasn’t increased or decreased. Smokers carrying751Glnallele made the risk of liver cancer increase1.58times. Among smoking people with liver cancers,28%ofthem were caused by the interactions of two factors (RERI=1.58, S=1.53, API=0.28). Drinkers carrying751Gln allele made the risk of developing gastric cancer and liver cancer increase1.61times and2.14times respectively.26%of drinkers with gastric cancers and40%of drinkers with liver cancers werecaused by the interactions of two factors (RERI=1.61, S=1.59, API=0.26; RERI=2.14, S=1.94, API=0.40).The concurrence of carrying751Gln allele and drinking didn’t increase or decrease the risk of developingcolorectal cancer (S≈1).Conclusion: The Lys751Gln genotype is risky genotype of gastrointestinal cancers including gastric cancer,liver cancer and colorectal cancer. Smokers carrying751Gln allele increased the risk of developing livercancer and had no correlations with risks of developing gastric cancer and colorectal cancer. Drinkerscarrying751Gln allele possibly increased the susceptibility of gastric cancer and liver cancer, and possiblyhad no correlations with risk of developing colorectal cancer. |
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