To Identify The Anatomical Structure Of GFP Mouse Brain By Stereostaxic Technique And Fluoroscence

Objective: To identify the anatomical structure of GFP mouse brain bystereostaxic technique and fluorescence.Methods: The GFP mouse brains are performed frozen section atcoronal/sagittal/horizonal position separately,then all sections are performedNissl staining for contrast.To define the anatomical structure with reference toatlas.Mark the coronal sections located at bregma and lambda from10adultGFP mouse brains,then produce frozen section,compare the difference amongindividuals and between mice and atlas. Make the razor blade slices of10separated brains at coronal and sagittal position,then make frozensection,compare the difference among individuals. To locate the anatomicalstructure of GFP brain in vitro.Results: Different structures can be identified by their distinctfluorescence intensity: the dense areas of nuclei, nissl bodies and nerve tractshow low fluorescence intensity, while the structures around the areas ofnuclei and nerve tract show high fluorescence intensity, such as, the plexiformlayer of olfactory bulb，the molecular layer of cerebella.The fluorescenceintensity is attenuated obviously after Nissl staining. The visualized structuralinformation observed under stereomicroscope is in accordance with thatviewed by fluoroscope and it also can been identified with reference to theatlas.The structure on the coronal sections located at bregma and lambda arein accordance but not the same to the atlas through the stereotaxis invivo.There are also no diversity among razor blade slices,so the stereotaxis in vitro is feasible,we can acquire the intrest site exactly and rapidly.Conclusion: To identify the anatomical structure of GFP mouse brain bystereostaxic technique and fluorescence.can serve as an experimental platformto be applied in fluorescence tracer experiments about the anatomic structurepositioning which are carried out widely.