Studies On The Pharmacy Of Stemonasessilifolia (Miq.) Miq

The study of this paper was mainly on t Stemonasessilifolia (Miq.) Miq. Based.on the literature review on the studies on chemical constituents, quality standard of total alkaloids, extraction and purification process of content, The purpose is to establish a quality standard of, Stemonasessilifolia (Miq.) Miq, and the preliminary study of medicinal, In order to effectively control the quality of medicinal materials, further understanding of its main pharmacodynamic material basis.In chemical constituent studies, two compounds were isolated from Stemonasessilifolia (Miq.) Miq. by determining physical constants、showing the color of the chemical reaction and using TLC、MS、1H-NMR、13C-NMR to analysis, oxotuberostemonine and bisdehydrotuberostemonine were identified. They were the highest concentration in Stemonasessilifolia (Miq.) Miq, which can be used as reference constituents in quality control of Stemonasessilifolia (Miq.) Miq and its preparation. The more systematic study of chemical constituents of Stemonasessilifolia (Miq.) Miq is necessary in future.In herbal medicine quality standard study, the study established the criteria of quality control of Stemonasessilifolia (Miq.) Miq, for it was not consummated in the former criterion which had only morphological and histological identification.The quality standard was incomplete which no TLC and content determination presented..TLC qualitative testing、determination of total alkaloids by ultra-voilet spectrophotometry and quantitative analysis by RP-HPLC were conducted to3samples from different habitats, using oxotuberostemonine and bisdehydrotuberostemonine as reference substance. These methods developed in this experiment can be used for the quality control of Stemonasessilifolia (Miq.) Miq. Extraction and purification process of total alkaloids in Stemonasessilifolia (Miq.) Miq testing study,Conducted a pilot study after its extraction and purification process parameters based on the total alkaloids of small scale experiments. Pilot study of the extraction process as follows:0.1mol/L,18times the amount of HCL, the speed of percolation7mL/min732strong acid cation exchange resin is the best adsorbent, ammonia alcohol ratio of1:1.5desorption elution oferect the total alkaloids dry extract. the total alkaloids purity is81.2%in this condition, provides a theoretical basis for the actual production.