| Objective：To research biological markers related with diagnosis andtreatment of pancreatic cancer and partly clarify pathogenesis, we observeddifferential proteins of pancreas cancer tissues from normal were byproteomics in human being and golden hamster.Methods：Golden hamster pancreatic cancer model was established, andthen total proteins of pancreatic cancer tissues and normal were respectivelyextracted from human beings and golden hamsters. 2-DE, PDQuest 7.1 andMALDI-TOF-TOF-MS were used to separate, obtain and identify differentialproteins, and part results were futher confirmed by immunohistochemicalstaining analysis.Results：(1) Twenty-one differentially expressed proteins were obtained asfollows: Nicotinamide N-methyltransferase (NNMT), Ferritin, glutathioneS-transferase M5, DNA topoisomerase, T-cell receptor beta chain, suppressorof IKK epsilon isoform1, transgelin, dimethylarginine dimethylamin-ohydrolase 1 (DDAH1), galactokinase 1, myosin；actin,α-enolase, annexinA4, keratin 8, thymidine kinase, PSMA1, carboxypeptidase B1, ribosomalprotein P1, phospholipaseA2, ankyrin3andphosphatidylethanolamine-bindingprotein.(2) Among these twenty-one proteins, two proteins were up-regulated [ Nicotinamide N-methyltransferase (NNMT) and Ferritin] (>4 folds), and theeight proteins were down-regulated [glutathione S-transferase M5, DNAtopoisomerase, T-cell receptor beta chain, suppressor of IKK epsilonisoform1, transgelin, dimethylarginine dimethylamin- ohydrolase 1 (DDAH1),galactokinase 1 and myosin] (>4 folds) in human pancreatic tumor tissues；The five proteins were up-regulated [actin,α-enolase, annexin A4, keratin 8and thymidine kinase] (>4 folds), and the seven proteins were down-regulated[PSMA1, carboxypeptidase B1, ribosomal protein P1, phospholipase A2,Ferritin, ankyrin 3 and phosphatidylethanolamine-binding protein] (>4 folds)in golden hamster pancreatic tumor tissues.(3) By immunohistochemistry staining analysis, ferritin was up-regulatedbut DNA topoisomerase, glutathione S-transferase M5 and myosin weredown-regulated in human tumor tissues.Conclusions：Nicotinamide N-methyltransferase, transgelin, DNAtopoisomerase, dimethylarginine dimethylamin- ohydrolase 1 (DDAH1),Ferritin，Annexin A4, ankyrin 3,α-enolase, carboxypeptidase B1 andribosomal protein P1 may be associate with infiltration and immigration oftumour cell, growth control, hypoxia endure, and angiopoiesis; glutathioneS-transferase M5，myosin，ribosomal protein P1 and PSMA1 may beinvolved in protein synthesis and degradation of cancer cells; T cell acceptorBeta–chain and suppressor of IKK epsilon isoform1 were related with signaltransduction; phosphatidylethanolamine-binding protein may be associatedwith construction and remodeling of cell membrane; annexin A4 and PSMA1may be new important biological markers related with diagnosis and/ortherapy of patients with pancreatic cancer... |
