Dtpa Inhibits Formation Of Staphylococcus Epidermidis Biofilm Associated With Prosthetic Joints:Relative Gene(AAP)Expressions

Objective:To investigate the inhibitory effect of DTPA on the formation of Staphylococcus epidermidis biofilm and the expressions of relative genes (AAP) in order to explore a new way to treat the infection induced by the bacteria.Methods:New zealand white rabbits were used to establish the animal model of prosthetic joints infection, Rabbits were randomly divided into the experimental group and control group. The bacteria were injected with0. lml1%DTPA into the knee of rabbits in experimental group; the rabbits were injected with bacteria in the control group, synovial fluid were extract in the2,4,6,8and16days. the expressions of AAP were detected by the way of fluorescent quantitation RT-PCR. Scanning electron microscopy and histological observations were observed.Results:DTPA can significantly inhibit the expression of AAP in Staphylococcus epidermidis. scanning electron microscopy found On the day of2,4,6,8,16the number of bacteria adhere to the prosthetic surface had decreased significantly in experimental group, compared with the control group. Conclusion:DTPA can inhibit the formation of the biofilm of Staphylococcus epidermidisthrough suppressing the relative genes.