| Objective:To detect the protein expressions of hfg12 (human fibrinogen like protein 2) from peripheral blood monocytes as well as Cerebrospinal fluid in patients with Cryptococcal meningitis and try to make clear the correlation and possible involvement between these diseases and the expression of hFgl2.Research hFgl2 prothrombin enzyme positive expression of mononuclear cells and Cerebrospinal fluid cryptococcal number、CSF protein and Cryptococcal capsular antigen quantitative to determine the correlation between the degree of The severity of meningitis, and understand the sensitivity of the reflection hFgl2 prothrombin enzyme expression determination of the clinical significance.Methods:1.Acquisition with complete clinical data of 20 cases with Cryptococcal meningitis(A) peripheral blood smear as experimental,20 cases of the healthy check-up peripheral blood smear as control group (B).2. Patients in group B were detected in the hospital diagnosed the first time the number of cerebrospinal fluid cryptococcal, cryptococcal fall by half the number of cryptococcal number of zero or cryptococcal collection of three peripheral blood in three different periods of culture-negative; And collection of matching patients with cryptococcal meningitis lumbar puncture the cerebrospinal fluid extract cells3.Application immunohistochemistry methods were used in the treatment of Cryptococcal meningitis group and the normal group peripheral blood monocytes.And using the two-step immunohistochemistry methods detected to Fgl2 protein expression in the collected in the Cerebrospinal fluid.4.The statistical data of expression was analysed by SPSS13.0.Results:1.Fgl2 proteins was detected in two groups of peripheral blood monocytes.Its expression of average gray level respectively was(Group A): (114.49±11.82), (Group B):(137.56±10.64), The two groups by statistical analysis, a statistically significant difference(P<0.05), Two sets of neutrophil cell expression hFgl2 average gray value (111.71±8.62), (143.56±12.57), by comparison, the difference was statistically significant (P<0.05).Prompt hFgl2 protein highly expressed in peripheral blood mononuclear cells and neutrophil cell of the cryptococcal meningitis group·2.hFgl2 protein expression in cryptococcal meningitis in cerebrospinal fluid mononuclear cells.. Average gray value of its expression with the negative control group was (103.33±8.07)、(147.25±9.72), The two groups by statistical analysis, a statistically significant difference(P<0.05), Positive and negative control group, the average gray value of neutrophils hFgl2 expression, respectively (115.44±8.67) (149.25±8.57), by comparison, the difference was statistically significant (P<0.05) single-core line-positive groupcells compared to the average gray value and to neutrophils hFgl2 protein expression was no significant difference (P> 0.05).Prompt hFgl2 protein highly expressed in the cryptococcal meningitis group cerebrospinal fluid mononuclear cells and neutrophil cell.3.the hFgl2 expression in Peripheral blood mononuclear cells and neutrophil cell.in three different periods of cryptococcal meningitis were positive, which Cerebrospinal fluid cryptococcal number of hospital diagnosed the first time, cryptococcosis decreased by half the number, cryptococcal number is zero or cryptococcal culture negative. Average gray value of its expression with the negative control group was (101.87±12.11) (106.63±8.38)、(118.37±11.26) respectively. There was significant difference in three groups (P<0.05). The average gray value of neutrophils hFgl2 expression, respectively (103.44±13.51) (107.31±8.87), (120.33±9.92), three groups, the difference was statistically significant (P<0.05). Further pairwise comparisons within the group differences were statistically significant (P<0.05). Expression by monocytes and neutrophils hFgl2 average gray value over the same period were compared, no statistically significant difference (P> 0.05).4. the hFgl2 expression in Cerebrospinal fluid mononuclear cells in three different periods of cryptococcal meningitis were positive, which Cerebrospinal fluid cryptococcal number of hospital diagnosed the first time, cryptococcosis decreased by half the number, cryptococcal number is zero or cryptococcal culture negative. Average gray value of its expression with the negative control group was (101.31±8.19)、(106.28±7.62)、(120.26±12.62) respectively. There was significant difference in three groups (P<0.05). The average gray value of neutrophils hFgl2 expression, respectively (103.23±9.08), (110.38±8.75), (121.06±10.43), three groups, the difference was statistically significant (P <0.05). Further pairwise comparisons within the group differences were statistically significant (P<0.05). Expression by monocytes and neutrophils hFgl2 average gray value over the same period were compared, no statistically significant difference (P> 0.05).Prompt the expression of hFgl2 in cryptococcal meningitis Cerebrospinal fluid’s positive values and disease severity of a positive correlation.5. In Peripheral blood mononuclear cells and neutrophil cell, the average gray value of hFg12 protein in Cryptococcal meningitis group and cerebrospinal fluid cryptococcal number was negatively correlated (P<0.05),it means the average gray value of hFgl2 protein with the cryptococcal meningitis group, the severity was negatively correlated, Cryptococcal meningitis in peripheral blood mononuclear cells of hFgl2 protein expression intensity of cerebrospinal fluid cryptococcal number into a positive correlation. There was no significant difference in the correlation of the average gray value of hFgl2 protein and CSF protein and cryptococcal capsular antigen quantitative(P>0.05).6. In cerebrospinal fluid mononuclear cells and neutrophil cell, the average gray value of hFgl2 protein in Cryptococcal meningitis group and cerebrospinal fluid cryptococcal number was negatively correlated (P<0.05), it means the average gray value of hFgl2 protein with the cryptococcal meningitis group, the severity was negatively correlated, Cryptococcal meningitis in Cerebrospinal fluid mononuclear cells of hFgl2 protein expression intensity of cerebrospinal fluid cryptococcal number into a positive correlation. There was no significant difference in the correlation of the average gray value of hFgl2 protein and CSF protein and cryptococcal capsular antigen quantitative(P>0.05).Conclusions:1. Fg12 protein can be in the human peripheral blood monocytes.2. hFgl2 protein can be expressed in cryptococcal meningitis in cerebrospinal fluid mononuclear cells· 3 the espression of hFgl2 protein reduced with the cryptococcal meningitis severity of the disease mitigate,and the espression of hFgl2 protein in peripheral blood and cerebrospinal fluid mononuclear cells showing the synchronization. prothrombinase hFgl2 is expected to become another evaluation of the degree of damage in the nervous system of patients with cryptococcal meningitis·... |
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