PD-1Expression Associated With The Pathology Of Chronic Hepatitis B Virus Infection

OBJECTIVES:To study the correlation between the expression of Programmed death1(PD-1) and the pathology of chronic hepatitis B, explore the role of PD-1in chronic HBV infection.METHODS:The liver biopsies were performed in all84patients with chronic hepatitis B, the liver tissues were10%formalin-fixed and paraffin-embedded sections were stained by HE, the hepatic inflammatory activity grade and fibrosis stage were evaluated. Immunohistochemical SP was used to detect the expression of PD-1in liver tissues. The positive expression means that red stain was found in five discontinuous visual fields under high magnification (400×).The results analyzed by Image Pro Plus6.0software.The expression intensity of PD-1in liver tissues were measured by Integral optical density (IOD) titres.RESULTS:The expression of PD-1was positively correlated with hepatic inflammatory activity grade and fibrosis stage, correlation coefficient are0.898(P<0.001)and0.700(P<0.01). There is significant differences of PD-1expression in each group of the hepatic inflammatory activity grade (P<0.05). The expression of PD-1is significantly different in each group of fibrosis stage (P<0.05),except S2 and S3, S3and S4(P>0.05)CONCLUSIONS:PD-1expression in liver tissues is positively correlated with the hepatic inflammatory activity grade and fibrosis stage. Detection of PD-lis worthy of evaluating hepatic inflammatory activity and fibrosis stage, meanwhile, it can provide valuable reference for assessment in hepatitic pathology.