CD147Mediated CypA A Study On Mechanisms For Promoting The Hepatocellular Carcinoma Proliferation

Objective: To study CypA on proliferation of hepatocellular carcinoma, to explore themechanism of CypA in promoting proliferation of Hepatocellular carcinoma.Methods:1.To Use RNA interference (RNAi) to build a low expression of CD147hepatocellularcarcinoma (7721-RNAi), CD147expression by Real-time PCR and Western blot analysis.2. With different concentrations of CypA, respectively, act on hepatocellular carcinoma(7721/RNAi), hepatocellular carcinoma7721and hepatocellular carcinoma (T7721), CCK-8assay to detect hepatocellular carcinoma proliferation.3.200ng/ml CypA act on Hepatocellular carcinoma (T7721), on different Time phase ofERK1/2of p38and its phosphorylated form of expression change by Western blot analysis.Results:1.7721/RNAi was successfully constructed, Real-time PCR and Western blot results show thelevel of CD147mRNA and protein expression significantly reduced compared to7721(P<0.05).2. Following with the increasing of CypA’s dose, OD value (450nm) increased with dosedependence. T7721proliferation was significantly higher than the7721and7721/RNAi.Diversity of CypA on proliferation in three types of hepatocellular carcinoma has statisticalsignificance(P<0.05).3. Stimulated by CypA at the dose of200ng/ml, the dose of phosphorylation of ERK1/2andp38MAPK signal protein increased relatively after five minutes(P<0.05).Conclusion:Proliferation of Hepatocellular carcinoma is promoted by CypA combined with its receptorCD147. And the effect of promoting proliferation of Hepatocellular carcinoma is most probablyassociated with the activity of endocellular ERK1/2and p38MAPK signaling molecules inducedby CypA.