Influnce Of The FLNa In Proliferation Inhibition Of Breast Cancer Cells Induced By Irradiation

In recent years, the morbidity of cancer increase gradually in ourcountry.Each year about2.6million newly increased,about1.8million deaths.Serious threat the life health of the residents. Among them, breast cancer is thehighest incidence of women malignant tumor. There are more than five millionpatients every year in the global. the death toll to Four hundred thousand. Withsurgery, radiotherapy,chemotherapy and hormone therapy treatment as the maintreatment method. But for patients with advanced breast cancer after a longtime of chemotherapy lead to drug resistance, often lead to the treatmentdifficult to progress. All the tumor cells have the common characteristics thatthe cloned proliferation ability of tumor cells. The ultimate goal of the cancertreatment is to inhibitie the proliferation of the tumor cell.Curenetely, the research for FLNa increased gradually. FLNa isCytoskeleton protein,it combined with a variety of protein, participate in theproliferation,adhesion, migration and hit.Related data confirm that FLNaexpress in a wide variety of tumor tissue.the level of the FLNa can indicate theprognosis. FLNa also express in the breast cancer cells. There are reports thatthe melanoma cells which lack of FLNa are more sensitive to the DNA damagethat induced by the irradiation. At present, the influnce of the FLNa to theproliferation of breast cancer cells reported less.Objective: To research the influnce of the FLNa in proliferation inhibitionof breast cancer cells induced by irradiation.Method:1.cell experiment: Human breast cancer cell lines divied into the normolgroup and different doses of radiation group. MTT method to detect the cells proliferation rate. To research the influence of FLNa for breast cancer cellproliferation preliminary.2.Flow cytometry to detect the cell cycle: Human breast cancer cell linesdivied into the control group and eight Gy doses of irradiation group, at0h,8h,16h,24h the four time points after irradiation to collect cells for the cell cycledetection.3.Immune imprinting technology testing the expression of the cdc2phosphorylation: To test the cdc2phosphorylation of expression at the fourtime points as the above.Results：1. The MTT experimental results: The cell survival of the two kinds ofcells has no change.After the irradiation,the proliferation of two kinds of breastcancer cell are subject to the obvious inhibition.Comparied with theMDA-MB-231group, the cells inhibition rate of the MB231-KD group washigher, the difference was statistically significant.2. Flow cytometry results:The cell cycle distribution is roughly samebetween the two kinds of cells when we use the0Gy dose of the irradiation，butwhen we use the8Gy dose of the irradiation，the cycle distribution appearsobvious difference. Two kinds of cells are apparent G₂/M period blockphenomenon at the16hours after the irradiation. At the24hour time point,theMB231-KD cells still showd obvious G₂/M period block phenomenon,instead the MDA-MB-231cell back to normal cell cycle.3. Immune imprinting technology results: the phosphorylation level ofcdc2in the two kinds of cells has no obvious difference with no irradiation.Theexpression of phosphorylation cdc2of the two cells begin increased after8hours with8Gy doses, after24hours the expression of phosphorylation cdc2ofthe MDA-MB-231cells become to decrease,but it still present high expressionstate in the MB231-KD cells. Conclusions:1. FLNa has no influence to the proliferation in the two berast cancer cellsin vitro.2. FLNa can adjust the proliferation inhibition of the breast cancer cellsinduced by irradiation3. The influence of FLNa to the recovery of the cell cycle block is relevantto the phosphorylation of cdc2.