Experimental Study Of Function Of S100A4Protein And Effects Of Ginkgo Biloba Extract In Viral Myocarditis

Background: Viral myocarditis is a common disease in pediatrics. Thereare different clinical manifestations.Onset it may be asymptomatic, it may bequickly developed into acute heart failure. It is also one of the main reasons ofcausing dilated cardiomyopathy. Therefore, VMC is a important illness whichthreat to children’s health. Because VMC lacks diagnostic specificity index, thedomestic and foreign mainly focuses on the pathogenesis of VMC.Objective: To study the role of S100A4in the process of the occurrenceand development of VMC and the intervention effect of Ginkgo biloba extractin VMC.Methods: Balb/c mice was infected by coxsackievirus B3(CVB3) toestablish VMC model. The mice were divided into control group (n=30) andvirus group(n=50) and treatment group(n=40). At infected5,10,15and30days, the score for myocardial necrosis and cellular infiltration was examinedby HE staining. The area of positive Masson stained myocardium collagenfibers was measured, and collagen volume fraction (CVF) was measured. Thenthe level of serum creatine phosphokinase-MB (CKMB) was determined. Thelevels of CTGF and TGF-β1were detected by streptavidin peroxidaseimmunoperoxidase technique. Expression of S100A4and MMP-3weredetected with reverse transcription-polymerase chain reaction(RT-PCR). At thesame time, the correlations were analyzed.Results: VMC ouse model was successfully build after intraperitonealinjection of CVB33days; The level of CKMB peaked on day10, anddecreased aften then. CVF increased significantly in virus group. Measured bystreptavidin peroxidase immunoperoxidase technique, the levels of S100A4and MMP-3increased in virus group, there was significant difference comparedwith control group.The expression of S100A4of myocardial tissue in VMCmice is enhanced with the prolonged viral infection; The expression of S100A4and MMP-3was positively linear correlated(r-pearson=0.952, P <0.01); theexpression of CTGF was negatively correlated with CVF (r-pearson=0.957, P<0.01). Compared with VMC group, the treatment group mice whichapplication of Ginkgo biloba extracts interventions in the treatment has a highsurvival rate and mild myocardial inflammation and myocardial fibrosis.Conclusion: S100A4is related with the occurrence and development ofVMC. S100A4is involved in the process of myocardial fibrosis caused byVMC, and this effect is executed in help of MMP-3. Ginkgo biloba extract candecrease VMC mortality, myocardial ischemia, attenuates myocardialinflammation, reduce the degree of myocardial fibrosis.