Preparation And Property Of SF/PLLA Composite Tissue Engineering Fibrous Scaffold

Recently, tissue engineering, which applies methods from engineering and lifesciences to create arti cial constructs to direct tissue regeneration, has attracted manyscientists and surgeons with a hope to treat patients in a minimally invasive and lesspainful way. The core of tissue engineering: the complex three-dimensional space,namely the establishment of cells and biological materials with the vitality of livingtissue to the reconstruction of the lesion tissue morphology, structure and functionand to achieve a permanent alternative. Come up with a suitable tissue engineeringscaffolds become an important part of the development of tissue engineering.Therefore, it is an important work that looking for a good biocompatibility andbiodegradability scaffold material with a large three-dimensional porous.Electrospinning is a materials processing method that is used to fabricatecontinuous, ultra-fine fibers of nanoscale diameter from polymers in solution. Theapproach is versatile, inexpensive, scalable and reliable. Many different polymers,additional solutes and solvents are compatible with successful spinning. Differentphysical properties of the spun materials can be controlled. Applications of thematerials are wide-ranging, encompassing filtration devices, textiles, electrical andoptical components, and sensors. Arguably, one of the most promising applicationareas for modern electrospinning is biomedical materials.In this paper, tissue engineering is the theoretical basis, via the electrospinningmethod, we select wide range of applications of biomedical materials such aspolylactic acid,silk fibroin as raw materials, prepared silk fibroin/poly (lactic acid)composite nanofibers, and the properties are investigated in detail as follows:1. Discussed the factors of electrospinning, and successfully prepared theregenerated silk fibroin. We study the effects of solution concentration, spinningvoltage, humidity and solution surface tension on the fiber morphology. Within acertain range, the greater the concentration, the better the strength of the fiber; theaverage diameter of the fibers increases with the spinning voltage increasing; lowhumidity conditions conducive to spinning.2. Using the solvent TFA and HFIP, different combinations of silkfibroin/polylactic acid fiber membrane was prepared via electrospinning process. Themorphology of the SF/PLA fiber menbrane was measured by scanning electron microscopy. The mechanical properties, contact angle,solubility in water anddegradation were also measured. The results showed that all the ratio of the fibersmorphology are all smooth and flat, the fibrous scaffolds we prepared withthree-dimensional structure, high porosity and good pore connectivity, porosity up toabout90%. With the increase of poly-lactic acid, the diameter of Fibroin/PLAmaterial is gradually increases, the diameter distribution is gradually narrow. Fibermembrane tensile strength, modulus and elongation are larger, gradual increase in themechanical properties. Contact angle increases, the fiber membrane solvent loss rateis reducing. Degradation experiment results show that the regulation of silk fibroinand poly-lactic acid distribution ratio of different groups to achieve controlleddegradation of the fiber membrane.3. Via the PLLA join in, we solve the problem that a single component of silkfibroin fiber membrane is dissolved in the water and with poor mechanical properties,also the silk fibroin can ease the degradarion rate of PLLA scaffolds. Thecombination of these two materials to improve a single component of their ownshortcomings, prepared a superior performance of tissue engineering scaffolds.4. We do the degradation experiments with SF/PLLA nanofibers prepared bydifferent solvents of the different components, to master the nature of the materialdegradation, which has played a guiding role in its cell culture, animal experimentsand tissue engineering, the degradation time of material is controllable.In summary, electrospinning technology is a simple and effective way to preparefiber scaffolds, via adjusting the material ratio of blend spinning, We can get differentpore size, porosity and mechanical properties of scaffolds, we can obtain theadvantageous material. The introduction of specific beneficial elements in the fiber,can be modified to single component materials of their own shortcomings, thus spunout of the scaffold material with excellent mechanical properties, make it play animportant role in the future development of tissue engineering.