The Preliminary Study On Fabrication Of Tissue Engineering Scaffolds Based On Silk Fibroin/organic Polymer Loaded With Icariin Using Coaxial Electrospinning Technique

Interdiscipline developed rapidly in recent years, the vigorous development of tissue engineering is established on the basis of this. The scaffold material, one of the three elements of tissue engineering, Can provide the basis for cell adhesion, proliferation and differentiationAt the same time, the rapid developments of engineering, chemistry, physics provide the basic theory and technology to support the scaffold material.Structures of nano fiber scaffold materials and natural extracellular matrix (ECMs) are very similar, so that it has excellent biocompatibility and its physical and chemical properties are also exceedingly good enough, and has been the focus of research in recent years. Some research results have been applied to Biomedical Engineering. Coaxial electrospinning can be used for the preparation of nano fiber material. This technology can change the morphology, physicochemical properties and biological properties of fibres by changing spinning parametersAt the same time, the material has sustained release property, which can adjust the release rate of the carrying drug in unit time.There are many types of nanofiber bracket materials made by coaxial electrospinning, such as natural materials,polymer materials, metal materials, ceramic materials, composite materials etc.Silk fibroin (SF) is a kind of natural material, which has wide sources, easy processing, good biocompatibility; but a single SF, preparation of scaffold materials, has poor mechanical performance, and is easily soluble in water, rapidly degraded. The mechanical and biological performanceof poly sinistral lactic acid, poly(caprolactone (poly real (L-actic-co-epsilon-caprolactone), PLCL) are excellent, but the degradation rate of PLCL is relatively slow, and it is difficult to processing. So this research focus on blending SF, and polymer PLCL to make the scaffold material of shell materials. Icariin (ICA) as the main component of Chinese medicine epimedium can significantly promotes the proliferation and differentiation to bone of stem cells, based on the research, we put forward using coaxial electrostatic spinning technology to build core layer for the ICA, the shell as the SF/PLCL new 3 d nano fiber scaffold, TCM monomer as carrying and slow release of growth factors.This research blended SF and PLCL accoding to the following mass fractions: 0:100,30:70,50:50,70:30,100:0, and dissolved them in six fluorine isopropyl alcohol (HFIP) separately for the preparation of composite nano fiber scaffold, after which crosslinking for 48 hours with glutaraldehyde (GTA) steam. To test the morphology and the physical and chemical properties, sem images showed that the fiber materials had good morphology, diameter uniformity, and smooth surface; after crosslinking, fiber swelling rate was low, which can maintain the original form. Mechanical performance test showed that the pure SF had high brittleness and was easy to fracture, but the addition of PLCL greatly improved the mechanical properties of scaffold material. X-ray diffraction analysis showed that after crosslinking of blending materials, SF showed more stable performance, and the conformation of these two materials changed unobvioursly. Thermogravimetric analysis concluded that the material thermal stability was good, which conformed to the expectation of this experiment. Hydrophilic Angle images showed the hydrophilism of the material was suitable. Various performance tests found the best mass fraction of SF/PLCL was 30:70.Secondly, we use the material with the SF/PLCL fraction of 30:70 as the shell material for the preparation of composite nano fiber scaffold carrying the ICA through the coaxial electrostatic spinning. Appearance characteristics tests showed that the velocity ratio of the shell and the core layer solution needed to be controled under 4:1, and the nano fiber scaffold materials were in good condition, transmission electron microscopy (TEM) showed obvious core and shell structure, and thermogravimetric and hydrophilic angle test were in line with the experimental expectations, XRD test showed that the hydrophilism of the scaffold material is appropriate after cross-linking, and the conformation of the three kinds of material unchanged.All in all, ICA-SF/PLCL nano fiber scaffold materials ICA with slow-release ability were prepared by coaxial electrospinning technology, and this research showed that it had good physical and chemical performance, and had the potential of being one of the tissue engineering scaffold materials of periodontal regeneration.