Research Of The Association Of Herpes Simplex Virus And Adenovirus With Chronic Conjunctivitis

Chronic conjunctivitis is a common disorder in ophthalmology. The causes includeinfection (bacteria, virus, chlamydiaetc)and non-infection(mechanical, chemical,personal hygiene and other eye disease stimulants). The incidence of viral chronicconjunctivitis increased recently as a result of antibiotics and hormone abuse. It is reportedthat the major sign of viral chronic conjunctivitis is inferior pleural follicles. In order toexplore the percentage of （herpes simplex virus-1，HSV-1）and (Adenovirus, ADV)infection in chronic conjunctivitis in patients presents inferior pleural follicles. Wecompared normal patients with chronic conjunctivitis patients, which categorized into twosubgroups by the sign of inferior pleural follicles. Samples were collected, and PCR（Real-time fluorescence quantification PCR, FQ-PCR) were used to detect the infectionpercentage of HSV-1and ADV.Purpose: To detect the infection percentage of HSV-1and ADV with real-timefluorescence quantification PCR between normal population and chronic conjunctivitispatients. And study the present rate of HSV-1and ADV in chronic conjunctivitis patientswhich manifested inferior pleural follicles and not manifest.Methods: Conjunctiva swab was collected from102patients diagnosed chronicconjunctivitis, another30swab were collected from normal population. FQ-PCR was usedto detect the infection percentage of HSV-1and ADV in the above groups.Viral infectionproportion was studied in chronic conjunctivitis. We categorized viral chronicconjunctivitis into two subgroups by the sign of inferior pleural follicles: with (n=59) andwithout (n=43). The infection proportion of HSV-1and ADV were determined by FQ-PCRin the two subgroups. Presents level of HSV-1and ADV in the subgroups were explored,and association between presents of inferior pleural follicles and different viral infectionwere analyzed. All the data were analyzed with SPSS l3.0.Results:①HSV-1positive percentage in chronic conjunctivitis patientsis23.53%(24/102), compared with HSV-1positive percentage of3.33%(1/30) in normalpopulation；HSV-1positive percentage is32.2%(19/59) in patients manifested inferiorpleural follicles, and11.63%(5/43)in patients without the manifestations；HSV-1infection positive percentage is significantly different (P<0.05) between the two subgroupscategorized by the sign of inferior pleural follicles.②ADV positive percentage in chronicconjunctivitis patients is27.45%（28/102）, compared with ADV positive percentage of6.67%(2/30)in normal population；ADV positive percentage is35.6%(21/59) in patientsmanifested inferior pleural follicles, and16.28%(7/43) in patients without themanifestations；ADV infection positive percentage is significantly different (P<0.05)between the two subgroups categorized by the sign of inferior pleural follicles.③Infectionproportion of HSV-1and ADV in chronic conjunctivitis patients is50.98%(52/102). Whilethe proportion is67.8%(40/59) in chronic conjunctivitis patients presented inferior pleuralfollicles.Conclusions：①There is a high percentage of viral infection involved by HSV-1andADV in chronic conjunctivitis.②Pleural follicle is associated with viral infection (HSV-1, ADV) inchronic conjunctivitis.