Polymorphisms Of The DNA Repair Genes XPD And XRCC1and The Susceptibility To Cervical Squamous Cell Carcinoma

Objective To investigate the relationship between susceptivity to cervical squamous cell carcinoma and polymorphisms of DNA repair enzyme genes XPD (Xeroderma pigmentosum complementary group D)、XRCC1(X-ray repair cross complementing group1) in Chinese population.Methods The Polymerase chain reaction and restriction fragment length polymorphisms (PCR-RFLP) was used to detectedthe susceptibility of cervical squamous cell carcinoma and polymorphisms of XPD-751and XRCC1-399in200patients and the same period200cases age-matched healthy individuals as controls.Results XRCC1-399genotypes in case and control groups were GG54%,67%, GA38%,27%, AA8%,6%(χ2=6.52, P<0.05) and allele distributions frequency G73%,83%, A27%,17%(χ2=6.30, P<0.05) frequency distribution of the differences in the satistically significant. The individuals who carried at least one A-allele (G/A or A/A) were more1.73-fold inclined to develop cervical squamous cell carcinoma compared with those who carried the G/G wild genotype of XRCC1-399genotype (OR=1.73,95%CI:1.15-2.56, P=0.008). No significant difference was found in the genotypes in case and control groups were AA86%,83.5%, AC14%,15.5%, CC1.0%((χ2=0.48, P>0.05) and allelic frequency distribution were A93%,91%,C7%,9%(χ2=0.84, P>0.05) of the polymorphisms in the XPD-751gene between in the groups was statistically significant.Conclusion1.These results suggest that the DNA repair enzyme gene polymorphism in XRCC1codon399may be associated with the development of cervical squamous cell carcinoma.2. No associated with the DNA repair enzyme gene polymorphisms in XPD codon751and the development of cervical squamous cell carcinoma.