| Objective:Discussion Zeqi’s monomer Quercetin, Gallic acid of the ALC of GuoYi master experience prescriptions inhibit liver cancer and promote in cell apoptosis of the role of mechanism.Methods:In vitro liver cancer cells SMMC-7721,cisplatin for positive group,observe different concentration Quercetin,Gallic acid in cell apoptosis role and its related mechanism.By MTT method to detect Quercetin,Gallic acid on the influence of the cell growth,and observed in different time points cells form;Use of flow cytometric detection after cell apoptosis of drugs and cell cycle distribution;Use RT-PCR and Western Blot method to detect monomer to SMMC-7721cells PI3K/AKT signaling pathways related apoptosis the influence of protein expression.Results:Quercetin,Gallic acid to human liver cancer cells SMMC-7721value-added had significant inhibitory effect,show concentration dependence and time dependence.Streaming suggests,Quercetin can make the SMMC-7721in cell cycle block G0/G1phase,and the apoptosis rate increased with the concentration;Gallic acid,can make the SMMC-7721in cell cycle block G0/G1phase,apoptosis rate of drug concentration increases with the increasing trend is not significant.Cell morphology,drug group of cells present irregular form,or even go round,the volume is narrow,the cellular structure no control group closely,some cells appear even fall off,floating phenomenon.RT-PCR,the Western Blot apoptosis related protein expression check showed that the expression of the drug group AKT inhibition,PTEN,Caspase9protein expression rate with the drug concentration increased.Conclusion:Quercetin,Gallic acid,all can induce human liver cell apoptosis SMMC-7721,its mechanism may be to block the cell cycle in G0/G1phase,the PTEN gene expression AKT activation had inhibition,activation, Caspase9and promote cell apoptosis.P13K/AKT signal pathways of expression,may be Qucrcetin,Gallic acid induced liver cancer cells SMMC-7721apoptosis of one of the mechanism. |
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