Effects Of Electro-acupuncture Of Zusanli Points On The Survival Rate And Visceral Functional Index Of The Rat With Delayed Fluid Resuscitation After Fatal Hemorrhagic Shock

Objective:To investigate the effects of electro-acupuncture of Zusanli points on the survival rate and visceral functional indexes of the rats with delayed fluid resuscitation after fatal hemorrhagic shock and to investigate possible mechanisms.Methods:Sixty SD rats were subjected to45%blood volume loss. After hemorrhage, animals were randomly divided into five groups (n=12):group of hemorrhagic shock(HS), group of delayed fluid resuscitation with electro-acupuncture(EA/DFR),group of delayed fluid resuscitation with sham electro-acupuncture(SEA/DFR), groupof electro-acupuncture(EA),group of instant fluid resuscitation(IFR).Group of HSperformed hemorrhagic shock with no treatment,Electroacupuncture of Zusanli pointswas performed in group of EA/DFR immediately after blood loss(electroacupuncturedfor40minutes,4Hz,4V),while electroacupuncture of non-channel points was conductedin group of SEA/DFR and the rest operation was similar to that for group ofEA/DFR.EA/DFR group and SEA/DFR group were both performed delayed fluidresuscitation after blood loss for3hours. Group of EA only performedElectroacupuncture of Zusanli points after blood loss the operation was similar toEA/DFR group. Group of IFR performed fluid resuscitation after blood lossimmediately. The mean arterial pressure(MAP) and visceral functional parameters weremeasured before hemorrhage and3、12and24h after hemorrhage, Survival rate for24hafter hemorrhage were recorded; Blood lactic acid content, diamine oxidase(DAO), theintestinal mucosal blood flow and the hepatic blood flow were measured beforehemorrhage and3、12h after hemorrhage; The rate of tissue water content of liver andgut were measured12h after hemorrhage.Results:1.After24hours’ blood loss the survival rate of group of EA/DFR wereclose to IFR group（P>0.05）,while at the12th hour the survival rate of EA/DFR groupwere significant lower than that of IFR group;2.Rats in all groups showed significantreduction of MAP after blood loss. After blood loss for3hours MAP of EA/DFR groupand EA group were significant higher than that of SEA/DFR group(all P<0.01), MAP ofEA/DFR group was significant lower than IFR group (P<0.01) after blood loss for12hours, but blood loss after24h,MAP of EA/DFR group showed no significant differencewith the MAP of IFR group(P>0.05);3.Rats in all groups showed significant reductionof ALT,CK-MB and Cr after blood loss(all P<0.01). After blood loss for3hours,ALT,CK-MB and Cr of IFR group were significant lower than those of other groups (allP<0.05), ALT and CK-MB of EA/DFR group and EA group were significant lower thanthose of SEA/DFR group and HS group (all P<0.05).There were no significant difference of Cr in the four groups (all P>0.05). After blood loss for12hours and24hours, ALT,CK-MB and Cr of EA/DFR group showed no significant difference withthose of IFR group(all P>0.05).4. Blood lactic acid content and DAO of EA/DFR groupand EA group were significant higher than those of SEA/DFR group and HS group afterblood loss for3hours (all P<0.05). After blood loss for12hours, blood lactic acidcontent and DAO of EA/DFR group and IFR group were significant higher than thoseof other groups(P<0.05and P<0.01).5. After blood loss for12hours, the intestinalmucosal blood flow and the hepatic blood flow of EA/DFR group and IFR group weresignificant higher than those of other groups(All P<0.05).6. The rate of tissue watercontent of liver and gut of EA/DFR group and IFR group were significant lower thanthose of other groups(All P<0.05)Conclusion:1.Electroacupuncture of Zusanli points conduces to MAP enhancementof rats with delayed fluid resuscitation after hemorrhagic shock, alleviation of visceralfunctional damage and acidosis, and increase of survival rate at the early stage.2.Electro acupuncture of Zusanli points protects intestinal barrier and liver function byprotecting DAO and improving ischemia and edema of gut and liver, it also plays atargeted role in digestive organ, putting off the transferring of toxin and bacterium. Thismay be one of the mechanisms of EA at Zusanli points exerting therapeutical effect onhemorrhagic shock.