Goldmag^? Particles-Based Chemiluminescence Immunoassay For Detection Of Morphine

Drug abuse problem is one of the major issues facing the world today. Due to the impact of drug-related activities abroad, the drug-taking cases in China are increasing in rescent years. Drug abuse has posed a great threat not only to people’s lives and properties, but also to people’s health and safety. Meanwhile, drug abuse could corrupt social morality, and induce a large number of illegal and criminal activities, which has brought great harm to our society, economy, law and public health. Because morphine family drugs account for the largest class of drug-consumed markets in China, the development of a rapid, sensitive method for detecting morphine drugs has a far-reaching significance to carry out the anti-drug activities, and make the clinical research on the monitoring of drug metabolism in vivo for patients.Using GoldMag(?) microparticles as a solid-phase carrier, the immunoassay for detection of morphine combined with chemiluminescence detection system was established. The results showed that this method has a good linear relationship in the range of5ng/mL～1000ng/mL with the correlation coefficient0.9973; IC50was63ng/mL and the limit of detection was0.43ng/mL; The CV of intra-assay and inter-assay were2.86～5%and5.1～9.7%respectively, both meeting the national standards; The recovery rate was in the range of91%～103%; There is no cross reaction with other structure-like drugs, except for a little bit with codein and heroin. Furthermore, the accelerated destructive test at37℃indicated that this method is relatively stable.The GoldMag(?) particles-based chemiluminescence immunoassay for morphine is a more reliable method with the advantages of high sensitivity and specificity, wide linear range, and good stability, which is expected to play an important part in the anti-drug work and quantitative detection of morphine family drugs in the future.