Rosemary Tissue Culture And In Vivo Rosmarinic Acid And Carnosic Acid Content Determination

Rosemarinus officinalis L. is the deciduous broad-leaved trees which from Labistae of Labiatae rosemary perennial evergreen shrubs, originating in the central Mediterranean coast.And European, American, Chinese, and other regions have distribution. Rosemary antioxidant is the main component of CA, Rosemary phenol,Ursolic acid, Rosemary acid. So it has anti-inflammatory, antibacterial and antiviral, anti-tumor, immune modulation, inhibition of the formation of uric acid and other aspects of the pharmacological effect, mainly used in food preservation technology, cosmetics and medicine etc. Through many methods to gain more rosemary ingredients, such as by using more appropriate cultivation techniques, to grasp the correct recovery time and methods,more fully separating and extracting effective components and other measures to achieve the goal. This paper mainly studies the rosemary tissue culture technology, to pairs of axillary and terminal buds as the explants,6BA and IBA of different concentrations and ratios on bud differentiation, proliferation, elongation and rooting. To identify a set of rosemary, tissue culture, explant disinfection using5%sodium hypochlorite sterilization for4minutes, using MS as basic culture medium. culture medium pH5.8. The initial stage of training, culture conditions selection of growth regulation of hormone and hormone concentration for6-BA0.5mg/L, sucrose20g/L and agar6g/L. Axillary bud induction and differentiation culture stage culture conditions for6-BA0.2mg/L+sucrose20g/L+6.5g/L agar, rooting stage culture conditions:IBA0.5mg/L+suc20g/L+agar6.5g/L. And through the HPLC method for the detection of rosemary plantlets of RC and CA content, we determines HPLC testing conditions of the rosemary plantlets of RA and CA:Mobile phase:CA flow match ratio of methanol:water:phosphoric acid=85:15:0.1(V/V/V), RA flow match ratio of methanol:water:phosphoric acid=55:45:0.1(V/V/V), Flow:1ml/min, Detection of wavelength:RA detection wavelength330nm, CA detection wavelength230nm,Column temperature:25C.As a result of the growth conditions change, rosemary in the main antioxidant component RA and CA in plants content have changed too. Tissue culture conditions of two acid content is higher than the usual.