The Expression And Clinical Significance Of Cell Cycle Gene In Esophageal Squamous Cell Carcinoma

Objective: To research the expression and corelationship of cell cycle gene CDC6,CyclinG1and CyclinG2in esophageal squamous cell carcinoma(ESCC) forinvestigating their clinical significance in pathogenesis and progression of esophagealsquamous cell carcinoma.Methods:1. CDC6,CyclinG1and CyclinG2protein expression was detected byimmunohistochemistry in20normal esophageal epithelium,16cases of atypicalhyperplasia of esophageal tissues and60cases of esophageal squamous cell carcinomaof expression.Then their relationships with the histology,clinical stage and lymph nodemetastasis were analyzed.2. CDC6and CyclinG1mRNA expression was detected byRT-PCR in14patients with esophageal cancer and adjacent normal tissues.Then theirexpression of mRNA were analyzed.Results:1. Immunohistochemistry results showed that the positive rate of CDC6was5.0%(1/20),12.5%(2/16) and70.0%(42/60) in normal esophageal epithelium,atypicalhyperplasia and esophageal squamous cell carcinoma.There was significant statistical.Difference between normal esophageal epithelium,atypical hyperplasia andESCC(P<0.01).Its expression in normal esophageal epithelium and atypical hyperplasiawas no significant difference.The expression of CDC6in ESCC was relevant to degreeof tumor differentiation (P<0.05).The expression of CDC6in ESCC was irrelerant withgender,age,clinical stage and lymphatic metastasis.The positive rate of CyclinG1innormal esophageal epithelium,atypical hyperplasia and esophageal squamous cellcarcinoma were25.0%(5/20),31.2%(5/16) and61.7%(37/60) accordingly.There wassignificant statistical difference between normal esophageal epithelium,atypicalhyperplasia and ESCC(P<0.01).Its expression in normal esophageal epithelium andatypical hyperplasia was no significant difference.The expression of CyclinG1in ESCCwas relevant to lymphatic metastasis and degree of tumor differentiation (P<0.05).Theexpression of CyclinG1in ESCC was irrelerant with gender, age, and clinical stage.Thepositive rate of CyclinG2was90.0%(18/20),87.5%(14/16) and66.7%(40/60) in normalesophageal epithelium,atypical hyperplasia and esophageal squamous cell carcinoma.There was significant statistical difference between normal esophagealepithelium,atypical hyperplasia and ESCC(P<0.05).Its expression in normal esophageal epithelium and atypical hyperplasia was no significant difference.The expression ofCyclinG2in ESCC was relevant to lymphatic metastasis and degree of tumordifferentiation (P<0.05).The expression of CyclinG2in ESCC was irrelerant withgender,age,and clinical stage.Correlation analysis showed that there was negativecorrelation between CyclinG1and CyclinG2(r=-0.267,P=0.039). And there was nocorrelation between CDC6CyclinG1and CyclinG2(P>0.05).2.CDC6mRNA andCyclinG1mRNA both in the esophageal squamous cell carcinoma were higher than innormal esophageal mucosa,the difference was statistically significant (P <0.01).Conclusion:1. CDC6in esophageal squamous cell carcinoma and normal esophagealmucosa and dysplasia tissues are different, and the differentiation of esophagealsquamous cell carcinoma were correlated, suggesting that CDC6may be involved inesophageal squamous cell carcinoma, development, and can be used as esophagealEarly diagnosis of cancer molecular markers.2. CyclinG1in esophageal squamous cell carcinoma and normal esophageal mucosaand dysplasia tissues are different, and with tumor differentiation and lymph nodemetastasis, and its abnormal expression in esophageal squamous cell carcinoma, sincethe process of development and metastasis important role in clinical diagnosis as animportant indicator of prognosis in esophageal squamous cell carcinoma.3. CyclinG2in normal esophageal mucosa is highly expressed in esophageal squamouscell carcinoma and the expression of differentiation and lymph node metastasis wassignificantly negatively correlated, suggesting CyclinG2loss of expression can be usedas early diagnosis of esophageal squamous cell carcinoma and prognosis of newindicators.4. CyclinG1and CyclinG2in esophageal squamous cell carcinoma was significantlynegatively correlated with the expression of esophageal squamous cell carcinomaCyclinG1increased, CyclinG2expression was significantly decreased, suggestingCyclinG1and CyclinG2negative phase between the regulation of each other, theirmutual role in promoting the occurrence of esophageal squamous cell carcinomadevelopment.