The Expression Of CyclinB1 And CyclinG1 In Non-small Cell Lung Cancer

Objective: Lung cancer is one of the most common malignant tumors, and the number of small-cell lung cancer accounts for more than 85% of lung cancer which is serious hazardous to human health. The development and occurrence of the Non-small cell lung cancer (NSCLC) is a complex, multi-gene participation with multiple factors, including activating mutations in oncogenes and tumor. Inactivation of suppressor gene is the most important factor.The cell cycle is the completion of each cell going through the process of self-replication which refers to the cell from one division to the next , it often takes 10-48 hours to complete the cell cycle. The cell cycle is divided into four periods, which is named the G1 phase, S phase, G2 phase and M phase. The cell cycle proteins (Cyclins) is product of phase-specific gene expression in a short period.There are a variety of biological eukaryotic cells, and cyclin-dependent kinase (Cyclin-dependent kinases, CDKs) is in basic active subunit,the cell cycle control points (cell-cycle checkpoint) play a key role in this regulation.Clinical facts show that compared with advanced lung cancer,early lung cancer has a better result.So looking for a genetic marker for lung cancer is extremely important. It is also very important to find the activation and inactivation of tumor suppressor genes and lung cancer. The relationship between the formation of the establishment which is simple, convenient, high accuracy diagnosis and treatment. In recent years, the disorder found in cell cycle regulation is one of the important reasons for cancer. Cycling is one of the most important compartments in the cell cycle control. CyclinB1 is the important proteins during G2-M phase in the cell cycle and CyclinG1 is later identified as a new member of the cell cycle proteins, both of them are closely related to a variety of tumors, but in non-small cell lung cancer the cases are not yet fully clear. In this study, we are not only trying to find out a new indicator for Non-small cell cancer in diagnosis and prognosis, but also trying to provide a new target and a theoretical basis for the treatment of NSCLC.Methods: The tissues in the study were taken from the Second Hospital of Hebei Medical University and the Fourth Hospital of Hebei Medical University.The tissues were diagnosed by clinical pathology.Then we took the normal lung tissue as a control group. All the patients had no preoperative radiotherapy and chemotherapy, and we have used surgical resection and sleeve lobectomy for lung resection. There were no distant metastases confirmed in the operation and after the operation. We used application of immunohistochemical staining (SP method) to the nucleus and / or the cell that was pasted into a brown-yellow staining was positive. We selected the performances randomly in five non-overlapping high power fields, then we used Image-pro plus 6.0 image analysis software for analysis. Each slice was selected randomly in five high-power view (10×40), measured from the perspective of staining positive and average optical density for each area. We took the optical density of every five cases of vision and calculated the means of the measured values. Then the data was input in SPSS 17.0 system and tested by two-sample t test, single-factor analysis of variance and q test (SNK) etc.Results:1 The expression of Cyclin in tissues:1.1 The expression of CyclinB1 in tissues:In 45 patients with NSCLC , 35 cases of CyclinB1 showed positive expression (M = 77.7%);In the normal control group, 2 cases in the 10 cases showed positive expression(M = 20.0%). The positive rate of the experimental group was significantly higher than the normal control group (p <0.01); The positive expression rate of lung squamous cell carcinoma is 17/22 (M = 77.3%); The positive expression rate of lung adenocarcinoma is 10/13 (M = 76.9%); The positive expression rate of squamous cell- adenocarcinoma is 8 / 10 (M = 80.0%).There is no relationship between each two groups in positive rate.1.2 The expression of CyclinG1 in tissues:In 45 patients with non-small cell lung cancer cases, 38 cases of CyclinG1 showed positive expression (M = 84.4%); the normal control group, 3 cases in the 10 cases showed positive (M = 30.0%). The experimental group has significantly higher positive rate. (p <0.01); The positive expression rate of lung squamous cell carcinoma is 19/22 (M = 86.4%); The positive expression rate of lung adenocarcinoma is 12/13 (M = 92.3%); The positive expression rate of squamous cell- adenocarcinoma is 7 / 10 (M = 70.0%). The three groups are not quite relevant to each other.2 The expression of both proteins in two experimental group (over 60 years old group and 60 years of age group) is not relevant to the age and gender(p>0.05).3 Expression of CyclinB1 in non-small is significantly different in every two of the NSCLC groups.Expression of CyclinG1:the differences between the well differentiated group and moderately are significantly different (p <0.05),but it is not significantly different between moderately differentiated and poorly differentiated groups. (p> 0.05).Conclusion:1 CyclinB1 and CyclinG1 of non-small cell lung cancer in adults have over expression .Abnormal cell cycle distribution shows that the two proteins may be an impact in non-small cell lung cancer occurrence and development.2 CyclinB1 and CyclinG1 are related to the tumor tissue differentiation. But the two kinds of cell cycle protein is not related to patient's age, gender and pathological type.