Effects Of Paclitaxel On Expression Of Gene And Protein In Wnt/β-catenin Signaling Pathway

Objective: Wnt signaling has recently emerged as a critical pathway in lung carcinogenesisas already demonstrated in many cancers and particularly in colorectal cancer.As is widely known,paclitaxel and other anti-microtubule agents facilitate the formation of the microtubule and inhibit itdepolymerize, then the cell circle is inhibited in G2/M。Besides， a direct function of paclitaxel oncell signaling and gene expression activation apotposis genes and expression protein can induceapotposis。we want to know the relationship between paclitaxel and Wnt/β-catenin signalingpathway, our research is focus on the quantity change of protein and mRNA in Wnt/β-cateninsignaling pathway after we give paclitaxel and try to give the possible mechanism.Methods: Non-toxic different doses of paclitaxel affected human lung adenocarcinoma cellA549for48hours，The protein levels of β-catenin was analyzed by immunohistochemicalmethod；RT-PCR method was used to detect dkk-3and c-myc mRNA expression level of A549cellline treated by paclitaxel for48hours within different concentration.Results: The expresson of β-catenin protein and oneogene c-myc were all downregμlated inA549cells(P<0.05)after treatment with paclitaxel for48hours,but the expresson of antioneogenedkk-3was downregμlated (P<0.05).Conclusion: paclitaxel coμld inhibit proteinβ-catenin and induce dkk-3mRNA expressionof lung cancer cell line A549，block Wnt signal transducfion pathway and inhibit the expression ofdownstream target genes c-myc．Thereby effectively inhibit the proliferation of A549cells．...