Expression Of PCDGF In Nasopharyngeal Carcinoma And Theeffect Of Small Interfering Rna Against Proliferation Of Nasopharyngeal Carcinoma HNE-1Cell Line

Background: Nasopharyngeal carcinoma (NPC) is one of the mostcommon cancers in South-China. It has a High degree of malignancy,powerful invasion and metastasis. because of the diseased parts of thehidden, It can not be early diagnosed. The PC cell-derived growth factor(PCDGF) Also known as progranulin,acrogranin, Belong to the growthfactor family: granulin(GRN) which highly expressed in tumor includinghuman breast cancer, ovarian cancer, esophageal squamous cell carcinoma,non-small cell lung cancer and multiple myeloma. It Involved in the tumoroccurrence,development, proliferation, invasion and metastasis. Therefore,silencing of PCDGF expression in favor of the prevention and treatment oftumor proliferation and metastasis. RNA interference (RNAi) is some smalldouble-stranded RNA(dsRNA) which can be used to block the expressionof target genes. In this study, We use RNA interference technology tosilence the expression of PCDGF, and investigate the impact of thebiological behavior of nasopharyngeal carcinoma cell line HNE-1after transfection.Objective: To Explore the expressions of PC cell-derived growthfactor (PCDGF) in nasopharyngeal carcinoma. And also to study therelationship of PCDGF protein with clinicl pathological features. To studythe effect of small interfering RNA (siRNA) against PCDGF gene on theproliferation of nasopharyngeal carcinoma HNE-1cell line.Methods: Immunohistochemical staining was used to detect in53cases of nasopharyngeal carcinoma tissues and28cases of chronicinflammation nasopharyngeal tissues of PCDGF protein. We alsocombination of clinical and pathological data for statistical analysis of theresults. Using immunofluorescence Analysis of HNE-1cell line. Accordingto the PCDGF Gene design of two siRNA expression plasmids. Theplasmids were transferred into HNE-1cell line by lipofectamine TM2000.The expression of PCDGF mRNA and protein were analyzed by real-timepolymerase chain reaction (real-time PCR) and Western blotting. UsingMTT assay cell proliferation and flow cytometry (FCM) detect cell cycleflow cytometry.Results: PCDGF positive expression rate in53cases of thenasopharyngeal carcinoma tissue was82.02%(44/53), and the chronicinflammation nasopharyngeal was17.86%(5/28). There was significantdifference between the two groups（P<0.01）. Moreover, the expression ofPCDGF protein were closely related to invasion, lymph node metastasis and Pathological stage of nasopharyngeal carcinoma (P<0.05). But notrelated to sex (P>0.05). Two of the plasmids both can inhibite theexpressions of PCDGF and the siRNA-1was confirmed to have thestrongest silencing efficiency on PCDGF gene expression in HNE-1cells at48h after transfection. The inhibitory rate of PCDGF mRNA and proteinwere59.3%and57.6%,respectively. The inhibition of cell proliferationratio in HNE-1cells reached (37.07±12.4)%and Cells cycle arrested inG0/G1phase.Conclusion: Specifc siRNA can effectively silence the expression ofPCDGF gene and significant inhibition cell proliferation of HNE-1cell.